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ORIGINAL RESEARCH article

Front. Vet. Sci., 07 June 2021
Sec. Animal Nutrition and Metabolism
This article is part of the Research Topic Nutritional Requirements in Production Animals View all 8 articles

Metabolizable Protein: 2. Requirements for Maintenance in Growing Saanen Goats

\nAnaiane P. Souza,Anaiane P. Souza1,2Julin A. C. VargasJulián A. C. Vargas3Marcia H. M. R. FernandesMarcia H. M. R. Fernandes1Amlia K. AlmeidaAmélia K. Almeida4Kleber T. ResendeKleber T. Resende1Izabelle A. M. A. Teixeira
Izabelle A. M. A. Teixeira1*
  • 1Department of Animal Sciences, Universidade Estadual Paulista, São Paulo, Brazil
  • 2Institute of Studies of the Humid Tropic, Universidade Federal do Sul e Sudeste do Pará, Xinguara, Brazil
  • 3Department of Animal Science, Universidade Federal Rural da Amazônia, Parauapebas, Brazil
  • 4University of New England, Armidale, NSW, Australia

This study aimed to estimate the protein requirements for the maintenance of growing Saanen goats of different sexes from 5 to 45 kg of body weight (BW) using two methods and applying a meta-analysis. For this purpose, two datasets were used. One dataset was used to evaluate the effects of sex on the protein requirements for maintenance using the comparative slaughter technique. This dataset was composed of 185 individual records (80 intact males, 62 castrated males, and 43 females) from six studies. The other dataset was used to evaluate the effects of sex on the protein requirements for maintenance using the N balance method. This dataset was composed of 136 individual records (59 intact males, 43 castrated males, and 34 females) from six studies. All studies applied an experimental design that provided different levels of N intake and different levels of N retention, allowing the development of regression equations to predict the net protein requirement for maintenance (NPM) and the metabolizable protein (MP) requirements for maintenance (MPM) in Saanen goats. The efficiency of MP use for maintenance (kPM) was computed as NPM/MPM. The efficiency of MP use for gain (kPG) was calculated using the equation of daily protein retained against daily MP intake above maintenance. A meta-analysis was applied using the MIXED procedure of SAS, in which sex was considered a fixed effect, and blocks nested in the studies and goat sex were considered as random effects. The NPM did not differ between sexes, irrespective of the approach used. The daily NPM estimated was 1.23 g/kg0.75 BW when using the comparative slaughter technique, while it was 3.18 g/kg0.75 BW when using the N balance technique for growing Saanen goats. The MPM estimated was 3.8 g/kg0.75 BW, the kPM was 0.33, and the kPG was 0.52. We observed that the NPM when using the comparative slaughter technique in growing Saanen goats is lower than that recommended by the current small ruminant feeding systems; on the other hand, the MPM was similar to previous reports by the feeding systems. Sex did not affect the protein requirements for maintenance and the efficiencies of use of metabolizable protein.

Introduction

Appropriate estimates of the protein requirements for maintenance and growth in goats support the nutritionist's decisions to formulate diets for improving animal production. Besides, inadequate balancing of protein content in ruminant's diet stimulates fecal and urinary nitrogen (N) excretion, which contributes to acid deposition, eutrophication, climate change, and respiratory diseases in humans (1, 2). Hence, accurate information regarding the protein requirements of dairy goats and the factors that affect them is pivotal to accomplish efficient diet formulation from a sustainable standpoint. One of these factors is sex, which impacts the body protein content of growing dairy goats (3).

Protein requirements for maintenance of ruminants include endogenous urinary protein, endogenous fecal protein, and dermal protein losses (4). Based on it, the N balance has been the most commonly used method for measuring the protein losses related to maintenance (5). Another method used for estimating the protein requirements for maintenance has been the comparative slaughter technique, which is based on the differences in body composition of animals slaughtered at different weights and nutritional levels (68). Even though retained N, measured by N balance methods or slaughter techniques, should be similar because it refers to a similar concept, the literature suggests that protein requirements for maintenance estimated by N balance studies are greater than those reported by the comparative slaughter studies (5). However, the reasons of these differences have not been conclusive and may be attributed to changes in dietary and environmental conditions across studies. Moreover, it has not been conclusive if sex has influenced these differences as well.

Multiple studies were conducted at our institution to estimate the protein requirements for maintenance in dairy goats of different sexes. We pooled and analyzed them under two meta-analyses for estimating the protein requirements for the maintenance of Saanen goats using the N balance and comparative slaughter methods. Our hypothesis is that sex influences the protein requirements for maintenance, which would be similar regardless of method used. In this sense, this study aimed to estimate the protein requirements for maintenance of growing Saanen goats of different sexes from 5 to 45 kg of body weight (BW) using two different methods and applying a meta-analysis.

Materials and Methods

Ethics Statement

All procedures used in the individual studies followed the University's Animal Care Committee [Comissão de Ética e Bem-Estar Animal (CEBEA)], under protocols described in each published source.

Data Collection

A dataset that included general information (e.g., author name), qualifying (e.g., sex, level of intake, and block), and necessary quantitative data of body composition and intake was gathered for this study. Data from individual animals were obtained from six comparative slaughter studies evaluating growing Saanen goats of different sexes from 5 to 45 kg of BW (5, 913). Animal's age ranged from 20 to 432 days for all sexes. All six studies adopted a randomized block as experimental design, where each block was composed of three pair-fed goats within sex randomly allocated to one of three levels of intake (ad libitum; moderate restriction, 25 or 30% of feed restriction; and maintenance level, 50 or 60% of feed restriction). The daily intake of the restricted-fed goats within a block was determined by the dry matter intake (DMI) of the goat fed ad libitum within the same block on the previous day. The crude protein (CP) and metabolizable energy contents of solid diets fed ranged from 137 to 175 g/kg DM and 2.4 to 2.7 Mcal/kg DM, respectively; CP of milk ranged from 283 to 285 g/kg DM. Body and diet protein contents were obtained by N analysis via Dumas combustion using LECO FP-528LC (14).

The net protein requirement for maintenance (NPM) was calculated using the comparative slaughter technique (6) and the N balance method as described below. In both approaches, the NPM was estimated as the intercept of the linear regression of N retained against N intake (Equation 1) multiplied by 6.25.

N retainedijk=ai+bi×Nintakeijk+sj+zk(j)+eijkl    (1)

N retainedijk is the dependent variable for the lth animal of the ith sex in the jth study in the kth block; Nintakeijk is the independent variable for the lth animal of the ith sex in the jth study in the kth block; aj and bj are the parameters to be estimated for each of the i = 1, 2, 3 sexes; sj is the random effect of the jth study ~ N (0, σs2); zk(j) is the effect of kth block nested in study jth; and eijkl is the residual error ~N (0, σe2).

Comparative Slaughter Technique

The daily N retained was estimated using the differences between the final body N content at slaughter and the initial body N content. The initial body N content was calculated as follows: (1) initial empty BW (EBW) of the animals was predicted from initial BW using the equation described by Souza et al. (3); and (2) initial body N was predicted from initial EBW across all studies using allometric equations for body protein described by Souza et al. (3) using the animals fed ad libitum that were included herein. The summary statistics of the main variables of the dataset by sex were presented (Table 1).

TABLE 1
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Table 1. Summary of descriptive statistics of body composition and intake of Saanen goats used in the comparative slaughter technique.

For estimating NPM, using the comparative slaughter technique, we used data of 185 dairy goats (62 castrated males, 80 intact males, and 43 females). A linear regression of N retained in the daily gain (g of N/kg0.75 BW and g of N/kg0.75 EBW) on N intake (g of N/kg0.75 BW and g of N/kg0.75 EBW) was used to calculate the net N requirement for maintenance (Equation 1). The intercept of the regression (i.e., ai parameter) was assumed to be the endogenous and metabolic losses of N, which when multiplied by 6.25 is assumed to be the NPM.

N Balance Method

For estimating NPM using the N balance method, we used data of 136 dairy goats obtained from digestibility trials (Table 2; 43 castrated males, 59 intact males, and 34 females). The feed intake and feed refusals were recorded; and feces and urine were collected for a minimum period of 5 days after an adaptation period as detailed in the published sources. We adopted 0.018 g N/kg0.75 BW to dermal losses (15). The N retained in this method was obtained as the difference between N intake and N excreted (sum of fecal, urinary, and dermal N). Similar to the comparative slaughter technique, the intercept of the regression of N retained on N intake was assumed to be the endogenous and metabolic losses of N, which when multiplied by 6.25 is assumed to be the NPM (Equation 1).

TABLE 2
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Table 2. Summary of descriptive statistics of N balance in Saanen goats used in this study.

Metabolizable Protein Requirements

The metabolizable protein requirement for maintenance (MPM) was estimated from the regression of retained protein (g/kg0.75 BW and g/kg0.75 EBW; calculated using the comparative slaughter technique) against the metabolizable protein intake (MPI; Equation 2). The MPI (g/kg0.75 BW or g/kg0.75 EBW) was calculated based on the true digestible microbial protein synthesis (MPS) plus the digestible rumen non-degradable protein intake. The MPS was estimated based on the results of a complementary study developed by (16) using the following equation: [MPS (g/day) = 18.13 + 12.48 × MEI (Mcal/day)]. The metabolizable energy intake (MEI; Mcal/day) of each animal was calculated on a dataset including gross energy intake, total energy losses from feces, urine, and gaseous products (17). True fraction and digestibility of microbial protein were both considered 80% (18). The rumen non-degradable protein intake was estimated using the feed composition reported by NRC (1), and its intestinal digestibility adopted was 80%. The MPM was obtained by assuming the retained protein as equal to 0.

Protein retainedijk=ai+bi×MPIijk+sj+zk(j)+eijkl    (2)

Protein retainedijk is the dependent variable for the lth animal of the ith sex in the jth study in the kth block; MPIijk is MPI, the independent variable for the lth animal of the ith sex in the jth study in the kth block; aj and bj are the parameters to be estimated for each of the i = 1, 2, 3 sexes; sj is the random effect of the jth study ~ N (0, σs2); zk(j) is the effect of kth block nested in study jth; and eijkl is the residual error ~ N (0, σe2).

Efficiencies of Metabolizable Protein Use

With the use of the results of the comparative slaughter technique, the efficiency of metabolizable protein use for maintenance (kPM) was computed as NPM/MPM. The efficiency of metabolizable use for gain (kPG) was estimated using the equation of daily protein retained calculated in the comparative slaughter technique against daily MPI above maintenance. For this calculation, we used the MPM estimated using Equation (2). This regression was set with an intercept equal to 0.

Statistical Analysis

Statistical analysis in all models was performed using the MIXED procedure of SAS (9.4) software. A mixed model was used assuming sex (i.e., castrated male, intact male, and female) as a fixed effect, and the effect of block nested in study and sex as a random effect. Statistical significance was declared at P < 0.10. The slopes and intercepts of each equation were estimated using the ESTIMATE statement of the MIXED procedure in SAS.

The general statistical model used was as follows:

Yijk=a0i+a1Xijk+sj+zk(j)+eijkl    (3)

where Yijk is the dependent variable for the lth animal of the ith sex in the jth study in the kth block; Xijk is the independent variable for the lth animal of the ith sex in the jth study in the kth block; a0i and a1i are the parameters to be estimated for each of the i = 1, 2, 3 sexes; sj is the random effect of the jth study ~ N (0, σs2); zk(j) is the effect of kth block nested in study jth; eijkl is the residual error ~N (0, σe2).

Three CONTRAST statements were applied to conduct pairwise comparisons of sex. Furthermore, three CONTRAST statements were applied to conduct pairwise comparisons when the interaction between sex and BW or EBW was found to be significant, indicating that at least two slopes differed between sexes (19). Outliers were removed when their normalized residuals were >|3|. For the comparative slaughter technique, five data points were removed (two castrated males, two intact males, and one female). For the N balance method, five data points related to different animals were removed (two castrated males, one intact male, and two females). For estimating the MPM, two outliers were removed (one castrated male and one intact male).

Monte Carlo simulations were performed to obtain the lower 90% confidence interval (LCI) and upper 90% confidence interval (UCI) of the MPM estimates. We calculated 10,000 simulated values for each of these protein requirements with a multivariate normal distribution for the parameters and error estimates, using the algorithm reported by Fan et al. (20).

Results and Discussion

Net Protein Requirements for Maintenance

Using the comparative slaughter technique, we evaluated the relationship between N intake (g/kg0.75 BW) and N retained in tissues (g/kg0.75 BW) in Saanen goats (Figure 1; Equations 4–7); n = 180, σb:s2 = 0.0109, σe2 = 0.00655). The NPM (i.e., the intercept of this regression multiplied by 6.25) did not differ between sexes (P = 0.67), and the overall value was 197 mg of N/kg0.75 BW (at N intake = 0), which corresponds to a NPM of 1.23 g/kg0.75 BW.

Castrated male: N retained=-0.233(±0.0487)                                               +0.293(±0.0264)×N intake    (4)
Intact male: N retained=-0.182(±0.0431)                                         +0.334(±0.0241)×N intake    (5)
Female: N retained=-0.176(±0.0622)                                   +0.249(±0.0401)×N intake    (6)
All sexes: N retained=-0.197(±0.0300)                                   +0.292(±0.0179)×N intake    (7)

When this equation was scaled by metabolic EBW, the NPM (i.e., the intercept of this regression) also did not differ between sexes (P = 0.61). We presented the relationship between N intake (g/kg0.75 EBW) and N retained (g/kg0.75 EBW) in Saanen goats (Equations 8–11; n = 180, σb:s2 = 0.0144, σe2 = 0.00916). The overall value of NPM (i.e., the intercept of this regression multiplied by 6.25) was 1.46 g/kg0.75 EBW.

Castrated male: N retained=-0.281(±0.0576)                                               +0.298(±0.0268)×N intake    (8)
Intact male: N retained=-0.210(±0.0507)                                         +0.333(±0.0246)×N intake    (9)
Female: N retained=-0.211(±0.0738)                                 +0.255(±0.0417)×N intake    (10)
All sexes: N retained=-0.234(±0.0355)                                      +0.295(±0.0184)×N intake    (11)

Using the N balance approach, we evaluated a relationship between N intake (g/kg0.75 BW) and N retained in N Balance (g/kg0.75 BW) in Saanen goats (Figure 2; Equations 12–15); n = 131, σb:s2 = 0.0503, σe2 = 0.0482). The NPM (i.e., the intercept of this regression multiplied by 6.25) also did not differ between sexes (P = 0.38), and the overall value was 509 mg of N/kg0.75 BW (at N intake = 0), which corresponds to a NPM of 3.18 g/kg0.75 BW.

Castrated male: N retained=-0.653(±0.135)                                               +0.582(±0.0773)×N intake    (12)
Intact male:N retained=-0.525(±0.111)                                         +0.662(±0.0596)×N intake    (13)
Female: N retained=-0.348(±0.172)                                   +0.508(±0.121)×N intake    (14)
All sexes:N retained=-0.509(±0.0817)                                   +0.584(±0.0519)×N intake    (15)

When this equation was scaled by metabolic EBW, the NPM (i.e., the intercept of this regression) also did not differ between sexes (P = 0.36). We presented the relationship between N intake (g/kg0.75 EBW) and N retained (g/kg0.75 EBW) in Saanen goats (Equations 16–19; n = 131, σb:s2 = 0.0669, σe2 = 0.0647). The overall value of NPM (i.e., the intercept of this regression multiplied by 6.25) was 3.76 g/kg0.75 EBW.

Castrated male: N retained=-0.779(±0.159)                                               +0.591(±0.0786)×N intake    (16)
Intact male: N retained=-0.616(±0.124)                                         +0.664(±0.0557)×N intake    (17)
Female: N retained=-0.411(±0.202)                                   +0.514(±0.123)×N intake    (18)
All sexes: N retained=-0.602(±0.0952)                                   +0.589(±0.0522)×N intake    (19)

The estimates of NPM obtained using the comparative slaughter technique were ~50% lower than those obtained using the N balance method. It has been reported that N balance overestimates the values and results in a greater variation for the protein requirements for maintenance (5, 7). The overall value using the comparative slaughter technique was lower than that reported by AFRC [(4); 2.19 g/kg0.75 BW of NPM], where the system adopted a kPM of 1.0 to maintenance. The daily requirement reported by AFRC is based on the sum of basal endogenous losses of N, considering the urinary, fecal, and dermal N.

FIGURE 1
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Figure 1. Relationship between daily N retained (g/kg0.75 BW) and daily N intake (g/kg0.75 BW) of Saanen goats of different sexes using the comparative slaughter technique. For all animals: N retained = −0.197 (±0.0300) + 0.292 (±0.0179) × N intake. The estimated block nested to study variances (σb:s2) and the residual variances (σe2) were 0.0109 and 0.00655, respectively. The parameters of the equation did not differ between sexes (P = 0.67). The observations were adjusted for the study effect.

FIGURE 2
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Figure 2. Relationship between daily N retained (g/kg0.75 BW) and daily N intake (g/kg0.75 BW) of Saanen goats of different sexes using the N balance method. For all animals: N retained = −0.509 (±0.0817) + 0.584 (±0.0519) × N intake. The estimated block nested to study variances (σb:s2) and the residual variances (σe2) were 0.0503 and 0.0482, respectively. The parameters of the equation did not differ between sexes (P = 0.38). The observations were adjusted for the study effect.

In both approaches, sex did not affect the NPM. The digestibility coefficients for CP (g/kg of DM) calculated using the N balance dataset presented a mean value of 0.70 for all animals. Although females present less body protein than males in dairy goats at a given BW (3), those differences possibly did not affect the protein requirements for maintenance in this study. Considering the mature weight previously estimated for Saanen goats (21) where females reach chemical maturity at lower BW (26 kg EBW) than castrated males (34.9 kg EBW) and intact males (42.6 kg EBW), we noted that all intact males evaluated in this study did not reach maturity, although few castrated males and females reached mature weight. We suspect that no effect was observed on the NPM because, in general, all animals were in the growth phase (animal's age ranged from 20 to 432 days for all sexes). The body protein content reaches a plateau close to maturity, and the notable changes in body composition related to sex are consequences of the increase in fat deposition instead of protein synthesis in dairy goats (21). Possibly, we did not find a remarkable difference in the body protein to affect the protein requirements for the maintenance of the dairy goats of different sexes evaluated herein.

The NPM obtained using the comparative slaughter technique is similar to the values obtained by the independent studies used in this dataset, as expected, in a study evaluating goat kids [1.32 g/kg0.75 BW in Saanen kids from 5 to 20 kg of BW; (9)], as well as in late growth goats [1.46 g/kg0.75 BW in Saanen goats from 30 to 45 kg BW; (5)]. The age seems not to affect the protein requirements for maintenance, similar to that previously described in pregnant dairy goats [1.38 g/kg0.75 EBW; (7)].

Comparing our results with other genotypes, the overall value of NPM obtained herein (1.23 g/kg0.75 BW) is lower than the NPM obtained in an individual study for Boer goats when also using the comparative slaughter technique [2.04 g/kg0.75 of BW; (22)]. Considering these differences between genotype reported in NRC (1), the MPM estimated in Angora goats (3.35 g/kg0.75 BW) is greater than the estimates in meat, dairy, and indigenous goats [3.07 g/kg0.75 BW; (23, 24)]. Differences in body composition between dairy, meat, or fiber goats may affect the MPM of these animals. Although dairy goats present a heavier gastrointestinal tract than meat goats (25), they present lighter carcass as a consequence of less muscular growth than meat goats at the same age. This is a consequence of the genotypes selected for milk production. Based on that, we could infer that less body protein in the carcass of dairy biotype supports lower body protein losses and, consequently, lower protein requirements for maintenance in dairy goats compared with meat goats.

The remarkable difference between the methods used herein for estimating the protein requirements for maintenance raises previous studies that reported that N balance may overestimate the NPM in metabolism trials. Our results agree with other studies that also reported that N balance can lead to biases of overestimation of N retention (5, 26). Estimates of NPM by the comparative slaughter technique yielded more precise equations than those obtained from the N balance data, which can be verified by the dispersion of the points in the graphs presented herein and in the residual analysis. Similarly, in pregnant goats, a low error was also reported using the comparative slaughter technique, where this approach possibly gave a more reliable reference value in mature goats (7). Slaughter studies are costly, but they indicate a more reliable measure of protein retention (2). The protein retention measured under slaughter methods is directly calculated, where representative samples of body tissues are obtained. Although the N balance trials used in this dataset described an adequate measurement of fecal and urine excretion including urine acidification, it is still a short-term experiment, and it is possibly more susceptible to errors in sample processing and analysis (2). The overestimated losses in N balance may improperly overestimate the protein requirements for maintenance. Additionally, little is known about the metabolic process' influence on N recycling in the body, since short-term regulation as ureagenesis can be reflected in a variation that is not clearly understood (27). Body proteins constantly undergo breakdown and re-synthesis, but these aspects remain unclarified (27). Losses of N during balance trials possible occur through routes that are not considered. In addition, the microbial metabolic activity in the ruminant metabolism and its effect on subsequent processes in the deposition of N in body tissues make the study of N metabolism in ruminants challenging compared with non-ruminants (2).

Metabolizable Protein Requirements and Efficiencies of Protein Use

The MPM did not differ between sexes (P = 0.557). We presented the relationship between daily protein retained obtained in the comparative slaughter technique approach (g/kg0.75 BW) and the daily MPI (g/kg0.75 BW) in Saanen goats (Figure 3; Equations 20–23; n = 183; σb:s2 = 0.0891, σe2 = 0.212). The overall value of MPM was 3.8 g/kg0.75 BW. The LCI and UCI for MPM were 3.10 and 4.38 g/kg0.75 BW, respectively.

Castrated male: Protein retained=-2.055(±0.288)                                                     +0.528(±0.0409)×MPI    (20)
Intact male: Protein retained=-2.164(±0.231)                                               +0.588(±0.0299)×MPI    (21)
Female: Protein retained=-1.775(±0.278)                                            +0.460(±0.0424)×MPI    (22)
All sexes: Protein retained=-1.98(±0.154)                                            +0.525(±0.0220)×MPI    (23)

When this equation was scaled by metabolic EBW, the MPM (i.e., the intercept of this regression) also did not differ between sexes (P = 0.47). We presented the relationship between daily protein retained (g/kg0.75 EBW) and daily MPI (g/kg0.75 EBW) in Saanen goats (Equations 24–27; n = 183; σb:s2 = 0.119, σe2 = 0.292). The overall value of MPM was 4.4 g/kg0.75 EBW. The LCI and UCI for MPM were 3.60 and 5.10 g/kg0.75 EBW, respectively.

Castrated male: Protein retained=-2.499(±0.338)                                                     +0.539(±0.0411)×MPI    (24)
Intact male: Protein retained=-2.466(±0.269)                                               +0.582(±0.0301)×MPI    (25)
Female: Protein retained=-2.006(±0.323)                                         +0.453(±0.0428)×MPI    (26)
All sexes: Protein retained=-2.322(±0.180)                                         +0.525(±0.0222)×MPI    (27)

With the use of the relationship between NPM/MPM, the calculated kPM was 0.33 for all sexes (the LCI and UCI were 0.28 and 0.40, respectively).

FIGURE 3
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Figure 3. Relationship between daily protein retained (g/kg0.75 BW) and daily metabolizable protein (MP) intake (g/kg0.75 BW) of Saanen goats of different sexes. For all animals: protein retained = −1.98 (±0.154) + 0.525 (±0.0220) × MPI. The estimated block nested to study variances (σb:s2) and the residual variances (σe2) were 0.0891 and 0.212, respectively. The parameters of the equation did not differ between sexes (P = 0.55). The observations were adjusted for the study effect.

The MPM estimated in this study was similar to that recommended by NRC (1) of 3.07 g/kg MPM kg0.75 BW. However, there is a noticeable difference between the kPM estimated in our study and the ones reported by the feeding systems. The kPM computed herein varied from 0.28 to 0.4, whereas values of 0.67 and 1.0 are reported by the most applied feeding systems (1, 4). The NRC (1) refers kPM as 1.0. According to this feeding system (1), the kPM value must be evaluated under distinct situations, for instance, in the well-fed state, or under low nutrient intake with tissue mobilization. To our knowledge, considering 100% efficiency is overestimated. Our findings suggest that knowledge about MP efficiency still deserves further attention, mainly about the estimative of MP and the range of MP levels in different situations.

When applying the equation of daily protein retained against daily MPI above maintenance (g/kg0.75 EBW), the equation did not differ between sexes (P = 0.82) and the overall kPG was 0.52 in Saanen goats (Equations 28–31; n = 183; σb:s2 = 0.119, σe2 = 0.292). The value of kPG for castrated males, intact males, and females was 0.54 (±0.0411), 0.58 (±0.0301), and 0.45 (±0.0428), respectively.

Castrated male: Protein retained=0.539(±0.0411)×MPI    (28)
Intact male: Protein retained=0.582(±0.0301)×MPI    (29)
Female: Protein retained=0.453(±0.0428)×MPI    (30)
All sexes: Protein retained=0.525(±0.0222)×MPI    (31)

The overall kPG calculated (0.52 ± 0.0222) was close to the value reported to goats in AFRC (4) (0.59) but lower than that adopted by SCA (28) and NRC (1), in which both adopted 0.70. This value was also close to that of recent studies in dairy cows by Castro et al. (29) of 0.51. Because the differences in protein content in tissue gain possibly affect this efficiency for growth (1), we would expect different values of kPG between sexes. Although we presented the overall kPG and the variation of results did not allow a difference between sexes, we opted for reporting kPG for each sex, considering that nutritionists may strategically adopt one or other in different situations of animal production: castrated males, 0.54 (±0.0411); intact males, 0.58 (±0.0301); and females, 0.45 (±0.0428).

In bovines, feeding low protein diets has been discussed in the last years, where no differences in animal performance among Nellore bulls fed diets containing 10, 12, or 14% CP were detected (30). The main interest in feeding diets with less protein content is that it can reduce N input, improving N utilization efficiency, thereby reducing the environmental impact caused by N losses from manure (31). Our results of NPM suggest that these requirements for growing dairy goats could be lower than those preconized by the current feeding systems (1).

In conclusion, the current study evaluated the protein requirements for maintenance and efficiency of metabolizable protein use in growing Saanen goats of different sexes, using both the comparative slaughter technique and N balance method, under a meta-analytical approach. Based on our results, we suggest that there is no evidence that sex affects the protein requirements for maintenance and efficiencies of protein use. The equations reported herein may improve the accuracy of protein requirements values adopted to dairy goats, thereby reducing the cost of the diets, as well as the environmental and social impacts of animal production.

Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Ethics Statement

All procedures used in the individual studies were followed by the University's Animal Care Committee (Comissão de Ética e Bem-Estar Animal – CEBEA), under protocols described in each one of the published sources.

Author Contributions

AS, JV, MF, AA, and IT: data curation, conceptualization, methodology, writing—original draft, review, and editing. KR and IT: funding acquisition, conceptualization, supervision, and project administration. All authors contributed to the article and approved the submitted version.

Funding

The authors thank the São Paulo Research Foundation (FAPESP, Grant #2014/14734-9, Brazil) and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil) for the financial support. This work was also financed in part by The Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior—Brasil (CAPES)-finance code 001.

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors are thankful to Dr. Normand St-Pierre from The Ohio State University for the advice regarding the statistics.

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Keywords: comparative slaughter, metabolism, N balance, ruminant, sex, efficiency of use, dairy goat

Citation: Souza AP, Vargas JAC, Fernandes MHMR, Almeida AK, Resende KT and Teixeira IAMA (2021) Metabolizable Protein: 2. Requirements for Maintenance in Growing Saanen Goats. Front. Vet. Sci. 8:650203. doi: 10.3389/fvets.2021.650203

Received: 06 January 2021; Accepted: 27 April 2021;
Published: 07 June 2021.

Edited by:

Polyana Pizzi Rotta, Universidade Federal de Viçosa, Brazil

Reviewed by:

Siaka Seriba Diarra, University of the South Pacific, Fiji
Massimo Trabalza-Marinucci, University of Perugia, Italy

Copyright © 2021 Souza, Vargas, Fernandes, Almeida, Resende and Teixeira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Izabelle A. M. A. Teixeira, izabelle.teixeira@unesp.br

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