AUTHOR=Xiao Yan , Zhang Suling , Yan He , Geng Xiaolin , Wang Yanwei , Xu Xin , Wang Mengyue , Zhang Haohao , Huang Baicheng , Pang Wenqiang , Yang Ming , Tian Kegong TITLE=The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O JOURNAL=Frontiers in Veterinary Science VOLUME=8 YEAR=2021 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2021.633706 DOI=10.3389/fvets.2021.633706 ISSN=2297-1769 ABSTRACT=

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escherichia coli was achieved with an optimal tandem permutation (VP0–VP3–VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.