AUTHOR=Massimini Marcella , Dalle Vedove Elena , Bachetti Benedetta , Di Pierro Francesco , Ribecco Cataldo , D'Addario Claudio , Pucci Mariangela 

TITLE=Polyphenols and Cannabidiol Modulate Transcriptional Regulation of Th1/Th2 Inflammatory Genes Related to Canine Atopic Dermatitis

JOURNAL=Frontiers in Veterinary Science

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2021.606197

DOI=10.3389/fvets.2021.606197

ISSN=2297-1769

ABSTRACT=<p>Canine atopic dermatitis (AD) is a multifactorial allergic disease associated with immune and abnormal skin barrier dysfunction and it is one of the primary causes of pruritus. Using a novel <italic>in vitro</italic> model of AD, here we tried to revert the alteration of transcriptional regulation of AD canine key genes testing a nutraceutical mixture containing flavonoids, stilbene, and cannabinoids, which are already well-known for their applications within dermatology diseases. The nutraceutical mixture induced in inflamed cells a significant downregulation (<italic>p</italic> &lt; 0.05) of the gene expression of <italic>ccl2, ccl17</italic>, and <italic>tslp</italic> in keratinocytes and of <italic>ccl2, ccl17</italic>, and <italic>il31ra</italic> in monocytes. Consistent with the observed alterations of <italic>tslp, ccl2, ccl17</italic>, and <italic>il31ra</italic> messenger RNA (mRNA) levels, a significant increase (<italic>p</italic> &lt; 0.05) of DNA methylation at specific CpG sites on the gene regulatory regions was found. These results lay the foundation for the use of these natural bioactives in veterinary medicine and provide a model for deeper understanding of their mechanisms of action, with potential translation to human research.</p>