AUTHOR=Morrell Jane M. , Abraham Maria Celina TITLE=Semen Handling in South American Camelids: State of the Art JOURNAL=Frontiers in Veterinary Science VOLUME=7 YEAR=2020 URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.586858 DOI=10.3389/fvets.2020.586858 ISSN=2297-1769 ABSTRACT=

Reproductive biotechnologies such as artificial insemination could be very useful for South American camelids, allowing widespread use of semen from breeding males with desirable genetics. However, artificial insemination is not widely employed in these species and is considered to have low overall efficiency. This is due in part to incomplete knowledge about the physiology of conception in these species, and also to challenges presented by semen collection and handling. Several recent reviews have centered on female camelid reproduction; therefore, in this review, the focus is on semen handling. Various semen collection methods are presented. Different methods of reducing seminal viscosity are compared, such as needling, enzyme treatment, and colloid centrifugation. Use of enzymes remains controversial because of widely differing results among research groups. Colloid centrifugation, particularly single layer centrifugation, has proved to be successful in facilitating development of sperm handling techniques in dromedary camels, and has also been used with llama semen. Therefore, protocols for colloid centrifugation of alpaca semen could be developed in the future.