AUTHOR=Hu Renge , Wang Xue , Muhamamd Inam , Wang Yiming , Dong Wenlong , Zhang Haipeng , Wang Yu , Liu Shuming , Gao Yunhang , Kong Lingcong , Ma Hongxia 

TITLE=Biological Characteristics and Genetic Analysis of a Highly Pathogenic Proteus Mirabilis Strain Isolated From Dogs in China

JOURNAL=Frontiers in Veterinary Science

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.00589

DOI=10.3389/fvets.2020.00589

ISSN=2297-1769

ABSTRACT=<p>To evaluate the antimicrobial resistance and virulence gene characteristics of highly pathogenic <italic>Proteus mirabilis</italic>. In this study, we isolated <italic>P. mirabilis</italic> CC15031 from diarrhea dogs in China, tested the median lethal dose (LD<sub>50</sub>), and measured the minimum inhibitory concentration (MIC) of 10 different antibiotics commonly used in veterinary clinic. Meanwhile, we presented the complete genome sequence annotations to analyze the virulence and resistance formation mechanism. The results showed that the CC15031 presented relatively potent pathogenicity in mice (LD<sub>50</sub> = 0.57 × 10<sup>6</sup> CFU) and exhibited a high degree of resistance to all the tested antimicrobial agents. The CC15031 genome of 4,031,742 bp with 3,745 predicted genes had an average gene length of 917 bp and 38.99% guanine-cytosine content. A new variant of an integrative and conjugative element with a type IV secretion system (217,446 bp) conferring multidrug resistance was identified and characterized by structural analysis in CC15031. These data provide a foundation for understanding the genomic features and antimicrobial resistance mechanisms of this pathogen.</p>