AUTHOR=Xia Zemiao , Geng Haopeng , Cai Yuan , Wang Yaonan , Sun Daquan , Zhang Jian , Pan Zhiming , Jiao Xin'an , Geng Shizhong 

TITLE=A McAb-Based Direct Competitive ELISA to Detect O:9 Salmonella Infection in Chicken

JOURNAL=Frontiers in Veterinary Science

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.00324

DOI=10.3389/fvets.2020.00324

ISSN=2297-1769

ABSTRACT=<p><italic>Salmonella enteritidis</italic> and <italic>Salmonella pullorum</italic> belonging to Group O<sub>9</sub><italic>Salmonella</italic> are major causative agents of infectious diseases in chicken. O<sub>9</sub> antigen as a part of lipopolysaccharide (LPS) is a predominant detected target for <italic>Salmonella</italic> infection. To identify the infection, an anti-O<sub>9</sub> monoclonal antibody (McAb)-based direct competitive enzyme-linked assay (O<sub>9</sub> Dc-ELISA) was developed after constraints were optimized; the establishment and application of O<sub>9</sub> Dc-ELISA, compared to two commercial kits and plate agglutination test (PAT), showed that O<sub>9</sub> Dc-ELISA could screen out more positive samples than the PAT method could and produce the same agreement rates with commercial kits in terms of sensitivity in addition to strong specificity to clinical serum samples.</p>