AUTHOR=Li Ronald H. L. , Nguyen Nghi , Rosati Tommaso , Jandrey Karl 

TITLE=Assessment of P2Y12 Inhibition by Clopidogrel in Feline Platelets Using Flow Cytometry Quantification of Vasodilator-Stimulated Phosphoprotein Phosphorylation

JOURNAL=Frontiers in Veterinary Science

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.00267

DOI=10.3389/fvets.2020.00267

ISSN=2297-1769

ABSTRACT=<p>The primary objective of this study was to evaluate a novel flow cytometry-based assay of quantifying platelet phosphorylation of vasodilator-stimulated phosphoprotein (P-VASP) in cats that received clopidogrel treatment. Eight healthy cats received 18.75 mg PO q24h of clopidogrel for 7 days. Prior to and after clopidogrel treatment, blood was collected for ADP-induced light transmission aggregometry (LTA) and P-VASP measurement by flow cytometry. Flow cytometry measurement of P-VASP levels was used to derive platelet reactivity index (PRI) before and after clopidogrel treatment. Based on P-VASP and LTA findings, platelet response to ADP was significantly attenuated after 7 days of clopidogrel treatment. By eliciting the competing platelet pathways of P2Y<sub>12</sub> and cAMP using ADP and PGE<sub>1</sub>, respectively, ADP had no effect on P-VASP levels following clopidogrel treatment (<italic>p</italic> = 0.94). Clopidogrel also significantly decreased PRI from 28.84 ± 28.52% to 1.69 ± 12.39% (<italic>p</italic> = 0.0078). PRI on day 8 correlated moderately with the degree of slope inhibition on LTA (<italic>r</italic> = −0.4, <italic>p</italic> = 0.4). Flow cytometry analysis of P-VASP is effective at monitoring the inhibitory effects of clopidogrel on feline platelets.</p>