AUTHOR=Balseiro Ana , Prieto José Miguel , Álvarez Vega , Lesellier Sandrine , Davé Dipesh , Salguero Francisco J. , Sevilla Iker A. , Infantes-Lorenzo José Antonio , Garrido Joseba M. , Adriaensen Hans , Juste Ramón A. , Barral Marta 

TITLE=Protective Effect of Oral BCG and Inactivated Mycobacterium bovis Vaccines in European Badgers (Meles meles) Experimentally Infected With M. bovis

JOURNAL=Frontiers in Veterinary Science

VOLUME=7

YEAR=2020

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.00041

DOI=10.3389/fvets.2020.00041

ISSN=2297-1769

ABSTRACT=<p>In Europe, badgers (<italic>Meles meles</italic>) are recognized as major tuberculosis (TB) reservoir hosts with the potential to transmit infection to associated cattle herds. Recent studies in Spain have demonstrated that vaccination with a heat-inactivated <italic>Mycobacterium bovis</italic> vaccine (HIMB) successfully protects captive wild boar and red deer against progressive disease. The aim of this study was to evaluate the efficacy of two oral vaccines against TB in a badger model: the live-attenuated <italic>M. bovis</italic> bacillus Calmette-Guérin BCG vaccine (Danish strain) and a HIMB vaccine. Twenty-four badgers were separated in three treatment groups: oral vaccinated with live BCG (10<sup>8</sup> CFU, <italic>n</italic> = 5), oral vaccinated with HIMB (10<sup>7</sup> CFU<italic>, n</italic> = 7), and unvaccinated controls (<italic>n</italic> = 12). All badgers were experimentally infected with <italic>M. bovis</italic> (10<sup>3</sup> CFU) by the endobronchial route targeting the right middle lung lobe. Throughout the study, clinical, immunological, pathological, and bacteriological parameters of infection were measured. Both vaccines conferred protection against experimental TB in badger, as measured by a reduction of the severity and lesion volumes. Based on these data, HIMB vaccination appears to be a promising TB oral vaccine candidate for badgers in endemic countries.</p>