AUTHOR=Zakirova Elena Yu , Shalimov Dmitry V. , Garanina Ekaterina E. , Zhuravleva Margarita N. , Rutland Catrin S. , Rizvanov Albert A. 

TITLE=Use of Biologically Active 3D Matrix for Extensive Skin Defect Treatment in Veterinary Practice: Case Report

JOURNAL=Frontiers in Veterinary Science

VOLUME=6

YEAR=2019

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2019.00076

DOI=10.3389/fvets.2019.00076

ISSN=2297-1769

ABSTRACT=<p><bold>Objectives:</bold> Large full-thickness skin defects represent a serious veterinary problem.</p><p><bold>Methods:</bold> We have developed novel bioactive 3D-matrixes based on fibrin glue Tissucol (Baxter), containing the combination of the adenoviral constructs with genes vascular endothelial growth factor 165 (VEGF165) and fibroblast growth factor two (FGF2; construct Ad5-VEGF165 + Ad5-FGF2) or multipotent mesenchymal stem cells, genetically modified with these constructs.</p><p><bold>Results:</bold><italic>In vitro</italic> studies confirmed the biosynthesis of VEGF165 and FGF2 mRNA in the transduced cells. Ad5-VEGF165 + Ad5-FGF2- transduced multipotent mesenchymal stem cells showed an enhanced capacity to form capillary-like tubes <italic>in vitro</italic>. Bioactive 3D-matrix application enhanced granulation tissue formation and epithelialization of non-healing, large bite wounds in a dog. Successful wound healing was observed with a positive clinical outcome for the canine patient. This research and application of regenerative gene therapy alongside a novel bioactive 3D-matrix shows promising clinical applications for the future in both dogs and other mammals including humans.</p>