AUTHOR=Cervantes-Valencia María Eugenia , Hermosilla Carlos , Alcalá-Canto Yazmín , Tapia Graciela , Taubert Anja , Silva Liliana M. R. 

TITLE=Antiparasitic Efficacy of Curcumin Against Besnoitia besnoiti Tachyzoites in vitro

JOURNAL=Frontiers in Veterinary Science

VOLUME=5

YEAR=2019

URL=https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2018.00333

DOI=10.3389/fvets.2018.00333

ISSN=2297-1769

ABSTRACT=<p><italic>Besnoitia besnoiti</italic> is the causative agent of bovine besnoitiosis. <italic>B. besnoiti</italic> infections lead to reduced fertility and productivity in cattle causing high economic losses, not only in Europe, but also in Asia and Africa. Mild to severe clinical signs, such as anasarca, oedema, orchitis, hyperkeratosis, and characteristic skin and mucosal cysts, are due to <italic>B. besnoiti</italic> tachyzoite and bradyzoite replication in intermediate host tissues. So far, there are no commercially available effective drugs against this parasite. Curcumin, a polyphenolic compound from <italic>Curcuma longa</italic> rhizome is well-known for its antioxidant, anti-inflammatory, immunomodulatory and also anti-protozoan effects. Hence, the objective of this study was to evaluate the effects of curcumin on viability, motility, invasive capacity, and proliferation of <italic>B. besnoiti</italic> tachyzoites replicating in primary bovine umbilical vein endothelial cells (BUVEC) <italic>in vitro</italic>. Functional inhibition assays revealed that curcumin treatments reduce tachyzoite viability and induce lethal effects in up to 57% of tachyzoites (IC<sub>50</sub> in 5.93 μM). Referring to general motility, significant dose-dependent effects of curcumin treatments were observed. Interestingly, curcumin treatments only dampened helical gliding and twirling activities whilst longitudinal gliding motility was not significantly affected. In addition, curcumin pretreatments of tachyzoites resulted in a dose-dependent reduction of host cell invasion as detected by infections rates at 1 day p. i. These findings demonstrate feeding cattle with <italic>Curcuma longa</italic> rhizomes may represent a new strategy for besnoitiosis treatment.</p>