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METHODS article
Front. Trop. Dis
Sec. Neglected Tropical Diseases
Volume 5 - 2024 |
doi: 10.3389/fitd.2024.1350680
This article is part of the Research Topic Female Genital Schistosomiasis: Research Needed to Raise Awareness and Deliver Action View all 12 articles
A rapid DNA screening method using high-resolution melt analysis to detect putative Schistosoma haematobium and Schistosoma mattheei hybrids alongside other introgressing schistosomes
Provisionally accepted- 1 Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
- 2 Kamuzu University of Health Sciences, Blantyre, Malawi
- 3 Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi
- 4 Semmelweis University, Budapest, Hungary
The phenomenon of hybridisation between Schistosoma species has gained a greater degree of significance since the WHO declared that schistosomiasis is to be eliminated, as a public health problem, by 2030. The role hybridisation plays in the transmission of disease is poorly understood and has the potential to complicate this elimination effort.A primary reason for this incomplete understanding of schistosome hybridisation is the lack of suitable, highthroughput and easily accessible methods capable of identifying the species-parentage of individual schistosomes.To address this resource gap, we present the development of a two-tube HRM assay capable of differentiating the species-parentage of schistosomes from a possible range of six species, namely: S. mattheei, S. curassoni, S. bovis, S. haematobium, S. mansoni and S. margrebowiei.
Keywords: HRM, Schistosomiasis hybrids, female genital schistosomiasis, qPCR, diagnostic
Received: 05 Dec 2023; Accepted: 15 Jul 2024.
Copyright: © 2024 Cunningham, Kayuni, Juhasz, Makaula, Lally, Namacha, Kapira, Chammudzi, Mainga, Jones, Archer, LaCourse, Musaya and Stothard. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Lucas J. Cunningham, Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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