Sara J Hutton Lauren Kashiwabara Erin Anderson Samreen Siddiqui Bryan Harper Stacey Harper Susanne M. Brander ^*

• Oregon State University, Corvallis, United States

Micro and nanoplastics (MNPs) are ubiquitous in the environment and have been detected in most ecosystems, including remove regions. The class of contaminants under the MNP umbrella is quite broad and encompasses variable polymer types, shapes, and sizes. Fibers are the most frequently detected in the environment, followed by fragments, but still represent only a small fraction of laboratory studies. Many toxicity studies have been done using polystyrene microbeads which represent neither the polymer nor shape most present in the environment. Additionally, most of these studies are done using virgin particles when the majority of MNP pollution is from secondary microplastics which have weathered and broken down over time. To address these data gaps, we exposed the model fish Inland Silverside, Menidia beryllina, for 21-days to micro and nano cryo-milled tire particles, micro and nano polylactic acid, and polyester microfibers, both weathered and unweathered treatments were tested. We evaluated the impacts of these particles on growth, behavior, and gene expression to compare the relative toxicities of the different particles. We found that overall, the nanoparticles and weathered fibers had the greatest effect on behavior and gene expression.Gene ontology analysis revealed strong evidence suggesting MNP exposure affected pathways involved in muscle contraction and function. Unweathered microfibers decreased growth which may be a result of food dilution. Our results also suggest that under weathering conditions polyester microfibers breakdown into smaller sizes and induce toxicity similar to nanoparticles. This study highlights the variable effects of MNPs in fish and emphasizes the importance of considering particle shape and size in toxicity studies.