Selim A. Alarape ¹ Azeezat O. Amusa ^2* Deborah A. Adeoye ¹ olanike k. adeyemo ¹

• ¹ University of Ibadan, Ibadan, Oyo, Nigeria
• ² Federal College Of Animal Health And Production Technology Ibadan, Ibadan, Nigeria

Geometric aquaculture growth has resulted in an exponential increase in the use of agrochemicals as herbicides for the aquaculture environment. This study was targeted at some toxicological (haematology and biochemistry) effects of glyphosate-based herbicides on non-target aquatic animals using Clarias gariepinus (C.G). METHOD: 80% of seventy five apparently healthy adult C.G (300g) locally sourced and acclimatized for two weeks were divided into four (4) treatment groups (fifteen per group; five per unit replicate) by simple randomization and labeled T0 (control), T1, T2, and T3 in three replicates. Four concentrations of Force up® [0ml, 0.15mls (0.003ml/L or 5.1mg/L), 0.225mls (0.0045ml/L or 7.65mg/L) and 0.3mls (0.006ml/L or 10.2mg/L) were added to 50L tank of water for T0, T1, T2 and T3 respectively. 5mls of blood was collected from all the fish in each treatment 96 hours post-exposure for measurement of blood parameters and biochemical indices using standard analytical methods and calculation of mean values.RESULT: The mean values of blood parameters: Packed Cell Volume (PCV), Haemoglobin concentration (Hb), Red Blood Cells (RBC), White Blood Cells (WBC) compared to the control group showed an initial increase at T1 but decreased as the glyphosate concentration increased at T2 and T3 respectively. Platelet mean values decreased at T1, increased at T2 and decreased at T3, while mean values of MCV, MCHC and MCH increased as the glyphosate concentration increased, though MCHC had a decrease at T2. Only platelet values were statistically significant with a p-value of <0.05 using ANOVA and post hoc (Tukey) tests.The biochemical indices showed a decrease in mean values of Aspartate Transaminase (AST), Blood Urea Nitrogen (BUN), Creatinine and Triglycerides at T1, increased values at T2 and decreased values at T3 while Total protein (g/dl), Cholesterol and Alanine Transaminase (ALT) and Alkaline Phosphatase (ALP) values showed increase at T1 and decrease at T2 and T3 respectively. All the values were not statistically significant using ANOVA with their p-values > 0.05. DISCUSSION: Itcan therefore be deduced that glyphosatebased herbicide (Force up®) has genotoxic, hepatotoxic as well as nephrotoxic effects on Clarias gariepinus even at sub-lethal doses with more adverse effects as the concentration increases.