AUTHOR=Longhin Eleonora Marta , El Yamani Naouale , Rundén-Pran Elise , Dusinska Maria 

TITLE=The alamar blue assay in the context of safety testing of nanomaterials

JOURNAL=Frontiers in Toxicology

VOLUME=4

YEAR=2022

URL=https://www.frontiersin.org/journals/toxicology/articles/10.3389/ftox.2022.981701

DOI=10.3389/ftox.2022.981701

ISSN=2673-3080

ABSTRACT=<p>The Alamar Blue (AB) assay is widely used to investigate cytotoxicity, cell proliferation and cellular metabolic activity within different fields of toxicology. The use of the assay with nanomaterials (NMs) entails specific aspects including the potential interference of NMs with the test. The procedure of the AB assay applied for testing NMs is described in detail and step-by-step, from NM preparation, cell exposure, inclusion of interference controls, to the analysis and interpretation of the results. Provided that the proper procedure is followed, and relevant controls are included, the AB assay is a reliable and high throughput test to evaluate the cytotoxicity/proliferation/metabolic response of cells exposed to NMs.</p>