AUTHOR=Cardoso Renato , Dusinska Maria , Collins Andrew , Manjanatha Mugimane , Pfuhler Stefan , Registre Marilyn , Elespuru Rosalie 

TITLE=In vivo Mammalian Alkaline Comet Assay: Method Adapted for Genotoxicity Assessment of Nanomaterials

JOURNAL=Frontiers in Toxicology

VOLUME=4

YEAR=2022

URL=https://www.frontiersin.org/journals/toxicology/articles/10.3389/ftox.2022.903896

DOI=10.3389/ftox.2022.903896

ISSN=2673-3080

ABSTRACT=<p>The <italic>in vivo</italic> Comet assay measures the generation of DNA strand breaks under conditions in which the DNA will unwind and migrate to the anode in an electrophoresis assay, producing comet-like figures. Measurements are on single cells, which allows the sampling of a diversity of cells and tissues for DNA damaging effects. The Comet assay is the most common <italic>in vivo</italic> method for genotoxicity assessment of nanomaterials (NM). The Method outlined here includes a recommended step-by-step approach, consistent with OECD 489, taking into consideration the issues impacting assessment of NM, including choice of cells or systems, handling of NM test articles, dose determination, assay methods and data assessment. This method is designed to be used along with the accompanying “Common Considerations” paper, which discusses issues common to any genotoxicity assay using NM as a test article.</p>