AUTHOR=Rawlings-Mortimer Florence , Lazari Alberto , Tisca Cristiana , Tachrount Mohamed , Martins-Bach Aurea B. , Miller Karla L. , Lerch Jason P. , Johansen-Berg Heidi 

TITLE=7,8-dihydroxyflavone enhances long-term spatial memory and alters brain volume in wildtype mice

JOURNAL=Frontiers in Systems Neuroscience

VOLUME=17

YEAR=2023

URL=https://www.frontiersin.org/journals/systems-neuroscience/articles/10.3389/fnsys.2023.1134594

DOI=10.3389/fnsys.2023.1134594

ISSN=1662-5137

ABSTRACT=<p><bold>Introduction:</bold> 7,8-dihydroxyflavone (7,8-DHF) is a low molecular weight compound that can cross the blood brain barrier and has been implicated in numerous functions and behaviours. It is thought to have neuroprotective capability and has been shown to alleviate symptoms in a wide range of diseases.</p><p><bold>Methods:</bold> 7,8-DHF was administered systemically to wildtype mice during Morris water maze training. Long-term spatial memory was assessed 28 days later. <italic>Ex-vivo</italic> T2-weighted (T2w) imaging was undertaken on a subset of these mice to assess brain-wide changes in volume.</p><p><bold>Results:</bold> We found that systemic 7,8-DHF administration during the training period enhanced spatial memory 28 days later. Volumetric changes were observed in numerous brain regions associated with a broad range of functions including cognition, sensory, and motor processing.</p><p><bold>Discussion:</bold> Our findings give the first whole brain overview of long-term anatomical changes following 7,8-DHF administration providing valuable information for assessing and understanding the widespread effects this drug has been shown to have in behaviour and disease.</p>