AUTHOR=Spalding Chloe , Shirgill Sandeep , Taylor Emma , Krachler Anne-Marie , Jabbari Sara TITLE=Mathematical Modelling of Pseudomonas aeruginosa L-forms Reveals Complex Interplay Between Host Defence Mechanisms and Putative Treatments JOURNAL=Frontiers in Systems Biology VOLUME=2 YEAR=2022 URL=https://www.frontiersin.org/journals/systems-biology/articles/10.3389/fsysb.2022.899990 DOI=10.3389/fsysb.2022.899990 ISSN=2674-0702 ABSTRACT=

The bacterium Pseudomonas aeruginosa has been shown to undergo a morphological transition akin to L-forms under exposure to antibiotics, a process which may contribute to persistent infections. With the further consideration of antibiotic-resistance mechanisms, this transition renders the design of effective treatment strategies challenging. Through a mathematical model, we illustrate that additionally incorporating the complexities of the host immune response can render somewhat surprising predictions from the simulations. In particular, scenarios arise whereby the addition of a treatment strategy to directly target the L-forms results in a worsened infection, while in others this treatment could turn an antibiotic-resistant infection from persistent to treatable. The study highlights the importance of understanding the in vivo interplay between immune cells and pathogens for successful treatment design.