AUTHOR=Guo Xianming , Qiao Qing , Jin Yutian , Lei Huaxiong , Guo Kunyao , Zhao Zhengxiong , Li Ping , Liu Aizhong , Sun Rui 

TITLE=Optimization of ultrasound-assisted extraction of two saponins from Paris polyphylla var. yunnanensis leaves using response surface methodology

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=8

YEAR=2024

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2024.1424285

DOI=10.3389/fsufs.2024.1424285

ISSN=2571-581X

ABSTRACT=<p><italic>Paris polyphylla</italic> var. <italic>yunnanensis</italic> is one of the famous Chinese herbs, in which two saponins (polyphyllin II and polyphyllin VII) have anticancer effects. The endangerment of <italic>Paris polyphylla</italic> var. <italic>yunnanensis</italic>, makes the study of optimizing the extraction of polyphyllin II and polyphyllin VII from the leaves of <italic>Paris polyphylla</italic> var. <italic>yunnanensis</italic> more important. The study established and optimized the process of ultrasound-assisted extraction for polyphyllin II and polyphyllin VII using the Box Behnken Design method of response surface methodology. The results showed that the optimum extraction conditions for polyphyllin II and polyphyllin VII are ethanol concentration of 73 and 70%, extraction temperature of 43 and 50°C, and number of extraction 3, respectively. Under the above conditions, the contents of polyphyllin II and polyphyllin VII were measured to be 6.427 and 19.015 mg/g (DW). The results showed that the experimental model fitted well, and the response surface methodology (RSM) was feasible to optimize the extraction process of polyphyllin II and polyphyllin VII from <italic>Paris polyphylla</italic> var. <italic>yunnanensis</italic> leaves. This method provides an effective approach for the comprehensive development and utilization of non-medicinal parts of <italic>Paris polyphylla</italic> var. <italic>yunnanensis</italic>.</p>