Lucerne (
In this study, we examined the effects of herbicide type [glyphosate and 2,4-D (G + 2), glyphosate and dicamba (G + D), 2,4-D and dicamba (2 + D), with water (W) and CT as controls] and concentration (100, 200, 300%) on various lucerne and soil properties.
Our findings revealed that G + 2 and 2 + D treatments significantly reduced the regreening rate, and the recommended herbicide concentration (100%) was sufficient for lucerne termination. CT and W treatments resulted in a higher soil pH compared to herbicide treatments. Moreover, herbicide treatments exhibited higher soil organic C (SOC) and total N (TN) levels than the CT treatment. The 2 + D treatment demonstrated a higher SOC content compared to the G + 2 treatment, while the 200 and 300% herbicide concentrations reduced SOC and TN levels. The G + 2 treatment had the highest soil ammonium nitrogen content (7.94 ± 1.45 mg kg−1), while the CT treatment showed the lowest (6.46 ± 1.54 mg kg−1). In conclusion, our study suggests that applying the recommended herbicide dosage (100%) effectively terminates lucerne grassland on the Loess Plateau of China without negatively impacting soil C and N storage. Finally, it is important to acknowledge that one-year trials at a single site have inherent limitations, and the findings should be considered cautiously when informing policy decisions.