AUTHOR=Mekala Krishna-Prashanth Ramesh , Pandit Saritha G. , Dhale Mohan A. 

TITLE=Bactericidal Effects of Exiguobacterium sp GM010 Pigment Against Food-Borne Pathogens

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=4

YEAR=2020

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2020.00142

DOI=10.3389/fsufs.2020.00142

ISSN=2571-581X

ABSTRACT=<p>Bacterium producing yellowish-orange pigment was identified (morphological, biochemical, and 16S rRNA) as <italic>Exiguobacterium</italic> sp GM010. The UV-visible spectrum of <italic>Exiguobacterium</italic> sp GM010 extract showing λ max at 465 nm revealed orange pigment characteristic. Pigment showed broad spectrum antibacterial action against gram positive and gram negative food-borne pathogens. The SYTO9 and propidium iodide (PI) staining revealed the cell membrane damage of food-borne pathogens under confocal laser scanning microscope (CLSM) indicating the bactericidal effect. This was evidenced by the fourier transform infrared (FTIR) spectrum, showing characteristic functional groups that mainly included hydroxyl, carbonyl, and carboxylic groups causing a system of delocalized electrons leading to destabilization of membrane and decrease in membrane potential that resulted in bactericidal effect. The pigment of <italic>Exiguobacterium</italic> sp GM010 were non-toxic against <italic>Artemia franciscana</italic> and can be a promising source to control the food-borne pathogens in food industries.</p>