AUTHOR=Ibáñez-Peinado Diana , Pina-Pérez Consuelo , García-Carrión Gema , Martínez Antonio , Rodrigo Dolores 

TITLE=In vivo Antimicrobial Activity Assessment of a Cauliflower By-Product Extract Against Salmonella Typhimurium

JOURNAL=Frontiers in Sustainable Food Systems

VOLUME=4

YEAR=2020

URL=https://www.frontiersin.org/journals/sustainable-food-systems/articles/10.3389/fsufs.2020.00008

DOI=10.3389/fsufs.2020.00008

ISSN=2571-581X

ABSTRACT=<p>The main objective of this work was to study the antimicrobial effect of a cauliflower by-product infusion into an affordable <italic>in vivo</italic> model (<italic>Caenorhabditis elegans</italic>). The infusion demonstrated some protective effect on non-infected and infected worms with <italic>Salmonella</italic> Typhimurium as indicated by higher survival percentile values (75, 50, 25, and 5% percentiles) as compared with those from worms unexposed to the infusion. The antimicrobial effect of the infusion was evaluated on <italic>Salmonella</italic> intestinal colonization of infected worms (24, 48, and 96 h post-infection). At 96 h post-infection, the concentration of <italic>Salmonella</italic> was reduced around 2 log cycles in infected cauliflower treated group (<italic>p</italic> &lt; 0.05) as compared with infected non-cauliflower group. Here we show that cauliflower by-products extend survival and have an antimicrobial effect in an <italic>in vivo</italic> nematode model, <italic>C. elegans</italic>, as a previous validation step to longer and costlier farm animal studies.</p>