AUTHOR=Wang Xiaoyang , Zhao Jie , Wang Xiaochuan , Zhang Jingjuan , Wang Yi , Wang Xinyue , Jia Shanshan , Shi Nian , Lu Meiqi , Su Hongxia , Zhang Jixun , Jiang Duyin 

TITLE=Bacterial cellulose membrane combined with BMSCs promotes wound healing by activating the notch signaling pathway

JOURNAL=Frontiers in Surgery

VOLUME=9

YEAR=2023

URL=https://www.frontiersin.org/journals/surgery/articles/10.3389/fsurg.2022.1027067

DOI=10.3389/fsurg.2022.1027067

ISSN=2296-875X

ABSTRACT=<sec><title>Objective</title><p>The bacterial cellulose membrane (BCM) has been widely studied and applied as a new biomaterial for wound healing, but causes pain with frequent dressing changes. Local application of bone marrow mesenchymal stem cells (BMSCs) requires a niche. Furthermore, the effect and mechanism of the BCM combined with BMSCs have not been reported.</p></sec><sec><title>Methods</title><p>Morphological and chemical identifications of BCMs were investigated by porosity analyses, scanning electron microscopy, and Fourier-transform infrared spectroscopy. Biological wound dressings (BWDs) were prepared by the BCM in combination with BMSCs. The biological effects of BWDs on human dermal fibroblast (HDF) and VEGF-A in human vascular endothelial cells (HuVECs) were detected <italic>in vitro</italic>, and the effect of BWDs on acute wounds in mice was detected <italic>in vivo</italic>. Collagen and angiogenesis were evaluated through hematoxylin–eosin staining and Masson staining. The expressions of <italic>COL-1</italic> and <italic>VEGF-A</italic> and the activation of the Notch signaling pathway <italic>in vivo</italic> and <italic>in vitro</italic> were detected by quantitative reverse-transcriptase polymerase chain reaction.</p></sec><sec><title>Results</title><p>The BCM had a nanoscale structure and provided a partial niche for the survival and proliferation of BMSCs. BWDs were successfully prepared and regulated the biological behaviors of wound healing-related cells <italic>in vitro</italic> and upregulated the expressions of <italic>COL-1</italic> in HDF and <italic>VEGF-A</italic> in HuVECs. BWDs promoted wound healing by increasing collagen type I synthesis and angiogenesis in acute wounds in mice.</p></sec><sec><title>Conclusions</title><p>BWDs prepared by the combination of nanomaterial BCMs and BMSCs facilitated acute wound healing, which may be regulated by activating the Notch signaling pathway.</p></sec>