AUTHOR=Bryant Saffron J. , Elbourne Aaron , Greaves Tamar L. , Bryant Gary TITLE=Applying soft matter techniques to solve challenges in cryopreservation JOURNAL=Frontiers in Soft Matter VOLUME=3 YEAR=2023 URL=https://www.frontiersin.org/journals/soft-matter/articles/10.3389/frsfm.2023.1219497 DOI=10.3389/frsfm.2023.1219497 ISSN=2813-0499 ABSTRACT=

Cryopreservation allows the long-term storage of biological material, and has become integral for numerous applications including assisted reproductive technologies, stem cell therapies, blood banking, and species preservation. However, there are still hundreds of cell types that cannot be cryopreserved, and no organs at all. The biggest challenge facing cryopreservation is the ongoing reliance on predominantly just two cryoprotectants (dimethylsulfoxide and glycerol) as well as physical limitations to heating and cooling rates. The field of soft matter can accelerate cryopreservation research by providing insights into the underlying mechanisms and interactions of cells, cryoprotectants, and solvents including the role of temperature. With techniques as far ranging as differential scanning calorimetry, atomic force microscopy, and neutron and X-ray scattering, it is possible to probe multiple length and time scales in order to identify and characterise important interactions. This in turn can lead to the development of new cryoprotectants, and new methods of heating/cooling which could overcome some of the current challenges of cryopreservation.