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ORIGINAL RESEARCH article

Front. Reprod. Health
Sec. Andrology
Volume 7 - 2025 | doi: 10.3389/frph.2025.1506117
This article is part of the Research Topic Oxidative Stress and Male Fertility View all 9 articles

Tackling somatic DNA contamination in sperm epigenetic studies

Provisionally accepted
Anamika Kumari Anamika Kumari Rajender Singh Rajender Singh *
  • Central Drug Research Institute (CSIR), Lucknow, India

The final, formatted version of the article will be published soon.

    Recent interest in sperm DNA methylation has stemmed from its implication in sperm DNA quality, sperm fertility, environmental toxicity, and transgenerational inheritance. Sperm DNA methylation data may be significantly affected by somatic DNA contamination, resulting in misleading conclusions. However, detecting and dealing with somatic cell contamination in semen samples can be a challenging task. In the present study, we have put together a detailed and robust plan to deal with somatic cell DNA contamination in sperm epigenetic studies in order to draw error-free scientific conclusions. This comprehensive plan, if followed, can ensure the complete elimination of the influence of somatic DNA contamination in sperm DNA methylation studies.

    Keywords: Sperm DNA, DNA Methylation, somatic cell contamination, sperm quality, epigenetic

    Received: 04 Oct 2024; Accepted: 13 Jan 2025.

    Copyright: © 2025 Kumari and Singh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Rajender Singh, Central Drug Research Institute (CSIR), Lucknow, India

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