Ju Keke ¹ Cao Weina ^1* Wu Ruikai ¹ Lu Yaoqin ^2*

• ¹ Shaanxi Provincial People's Hospital, Xi'an, Shaanxi Province, China
• ² Xinjiang Medical University, Ürümqi, Xinjiang Uyghur Region, China

Background：In recent years, the importance of biosafety research has garnered significant attention due to its critical implications for public health and safety. Biosafety Laboratory(BSL) personnel face numerous challenges as they work with high-risk pathogens, including high-pressure environments, stringent safety protocols, and the risk of infection. Research indicates that occupational stress and burnout significantly affect the physical and mental well-being of laboratory personnel, potentially diminishing their work efficiency and capabilities. This study aims to investigate the effects of occupational burnout and sleep quality on the work ability of BSL personnel in Xinjiang, ultimately providing valuable insights for enhancing biosafety and improving work efficiency.In July 2022, a cluster sampling method was employed to survey the staff of BSL in Xinjiang. The study utilized the Maslach Burnout Inventory, the Pittsburgh Sleep Quality Index, and the Work Ability Index to assess the levels of occupational burnout, sleep quality, and work ability among the BSL personnel. Statistical analyses were performed using R Studio 4.2.2 and AMOS 26.0. Through t-tests, analysis of variance, and logistic regression analyses, the study explored the current status and influencing factors of work ability among Xinjiang BSL personnel, as well as the interactive and mediating effects of occupational burnout and sleep quality on work ability.The prevalence of occupational burnout was 67.4%, while the prevalence of sleep disorders was 38.9%.Interaction analysis revealed that BSL personnel experiencing both occupational burnout and sleep disorders faced a risk of impaired work ability 21.43 times greater than those without burnout and with good sleep quality (OR = 21.43, 95%CI: 14.30-32.12). Structural equation modeling indicated that occupational burnout indirectly impacts work ability through its effect on sleep quality. The indirect effect was significant with a path coefficient of β = -0.28 (0.64 ×-0.43), P < 0.01.The overall work ability of staff in BSL in Xinjiang is relatively good. Occupational burnout and sleep quality are significant risk factors affecting the work ability of BSL personnel. Improving occupational burnout and sleep quality can enhance the work ability of BSL personnel both directly and indirectly.