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ORIGINAL RESEARCH article

Front. Public Health
Sec. Infectious Diseases: Epidemiology and Prevention
Volume 12 - 2024 | doi: 10.3389/fpubh.2024.1436648
This article is part of the Research Topic Biofilms in Healthcare Settings View all 5 articles

Antibiotic susceptibility of Pseudomonas aeruginosa in Saudi Arabia: A national antimicrobial resistance surveillance study

Provisionally accepted
Abrar K. Thabit Abrar K. Thabit 1*Ammar M. Alghamdi Ammar M. Alghamdi 1Musaab Y. Miaji Musaab Y. Miaji 1Feras S. Alharbi Feras S. Alharbi 1Anas F. Jawah Anas F. Jawah 1Fatimah Alturki Fatimah Alturki 2Nehal Hosin Nehal Hosin 3Mohammed Bazuqamah Mohammed Bazuqamah 4Masaad Almutairi Masaad Almutairi 5Hamad Alhamed Hamad Alhamed 6Alaa Elhendawy Alaa Elhendawy 7Dalya Atallah Dalya Atallah 8Abdulaziz A. Humadi Abdulaziz A. Humadi 9Khalid A. Alfifi Khalid A. Alfifi 9Khadija Alfadel Khadija Alfadel 10Khalid Eljaaly Khalid Eljaaly 1Mahmoud A. Elfaky Mahmoud A. Elfaky 1
  • 1 King Abdulaziz University, Jeddah, Saudi Arabia
  • 2 King Fahd University Hospital, Imam Abdulrahman Bin Faisal University, Al Khobar, Riyadh, Saudi Arabia
  • 3 Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
  • 4 King Khaled Hospital, Najran, Saudi Arabia
  • 5 Qassim University, Buraidah, Al-Qassim, Saudi Arabia
  • 6 King Fahd Specialist Hospital, buraydah, Saudi Arabia
  • 7 King Fahad Hospital, Madinah, Saudi Arabia
  • 8 King Abdul Aziz University Hospital, Jeddah, Makkah, Saudi Arabia
  • 9 King Fahad Specialist Hospital Dammam, Dammam, Saudi Arabia
  • 10 King Salman bin Abdulaziz Hospital, Riyadh, Saudi Arabia

The final, formatted version of the article will be published soon.

    Background: Pseudomonas aeruginosa is a common pathogen causing healthcare-associated infections. Most surveillance studies from Saudi Arabia that assessed the resistance by P. aeruginosa were conducted in single centers or did no't use the gold standard test broth microdilution (BMD), the gold standard test. This is the first national multicenter study to assess the resistance profiles of P. aeruginosa isolates in Saudi Arabia using BMD.Methods: Between 2022 and 2023, isolates from various infection sites were collected from 7 seven hospitals from 7in seven different regions of Saudi Arabia. The isolates were shipped to an academic microbiology lab, where their susceptibility was tested by BMD following Clinical Laboratory Standards Institute guidelines using Sensititre GNX3F plates. %sSusceptibility to each antibiotic, as well asand MIC50 and MIC90 were determined.Results: In total, 185 P. aeruginosa isolates were collected. Most isolates came from respiratory specimens (34.1%), followed by urine (21.1%) and skin/soft tissue (17.8%). The greatest highest susceptibility was to amikacin (76.8%). Concurrently, Ssusceptibility to meropenem was 52%, but it was 43.8% to colistin. While all P. aeruginosa isolates met the definition of multidrug-resistance, 41 (22.2%) met the definition ofwere difficult-to-treat and 10 (5.4%) were pandrug-resistant. Difficultto-treat isolates made up 30.3% of skin and soft tissue isolates, 25.4% of respiratory isolates, 21.7% of blood isolates, and 17.9% of urine isolates.P. aeruginosa showed demonstrated an unexpectedly high level of resistance to several commonly used antibiotics. Therefore, Aantimicrobial stewardship and infection control policies should be strictly enforced by hospitals across the country to optimize treatment, prevent the emergence and spread of resistant strains, and track resistance trends with local antibiograms.

    Keywords: Pseudomonas aeruginosa, antimicrobial resistance, Broth microdilution, surveillance, Saudi Arabia

    Received: 28 May 2024; Accepted: 16 Sep 2024.

    Copyright: © 2024 Thabit, Alghamdi, Miaji, Alharbi, Jawah, Alturki, Hosin, Bazuqamah, Almutairi, Alhamed, Elhendawy, Atallah, Humadi, Alfifi, Alfadel, Eljaaly and Elfaky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Abrar K. Thabit, King Abdulaziz University, Jeddah, Saudi Arabia

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