AUTHOR=Bai Yuanyuan , Shao Chunhong , Hao Yingying , Wang Yueling , Jin Yan 

TITLE=Using Whole Genome Sequencing to Trace, Control and Characterize a Hospital Infection of IMP-4-Producing Klebsiella pneumoniae ST2253 in a Neonatal Unit in a Tertiary Hospital, China

JOURNAL=Frontiers in Public Health

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2021.755252

DOI=10.3389/fpubh.2021.755252

ISSN=2296-2565

ABSTRACT=<p><bold>Background:</bold> The purpose of this study is to use whole genome sequencing (WGS) combined with epidemiological data to track a hospital infection of the carbapenem-resistant <italic>Klebsiella pneumoniae</italic> (CRKP), which affected 3 neonatal patients in the neonatal intensive care unit (NICU).</p><p><bold>Methods:</bold> The minimum inhibitory concentrations for the antimicrobial agents were determined according to the guidelines of the Clinical and Laboratory Standards Institute. Beta-lactamases were investigated using the polymerase chain reaction and DNA sequencing. The transferability of the plasmid was investigated by a conjugation experiment. The clonal relationships were evaluated using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). WGS and single nucleotide polymorphisms (SNPs) analysis were performed on the CRKP isolates to investigate how the infection might progress.</p><p><bold>Results:</bold> Nine CRKP isolates were obtained from the NICU, seven from three patients, one from a duster cloth and one from the hand of a nurse, they all harbored <italic>bla</italic>IMP-4. Other resistance genes including <italic>bla</italic>KPC-2, <italic>bla</italic>IMP-4, <italic>bla</italic>SHV-1, <italic>bla</italic>TEM-1, <italic>bla</italic>CTX-M-15, and <italic>bla</italic>DHA-1 were also detected. PFGE analysis showed that IMP-4-producing <italic>K. pneumoniae</italic> were clonally related, and MLST assigned them to a new sequence type 2253. The SNP variations throughout the genome divided the 9 strains into three clades. Clade 1 comprised 7 strains (K1- K2 and K4-K8), whereas clade 2 and 3 consisted of only one strain each: K3 and K9, respectively.The sputum isolate K3 from patient 3 was the most distinct one differing from the other eight isolates by 239-275 SNPs.</p><p><bold>Conclusions:</bold> This is a report of using WGS to track a hospital infecion of IMP-4-producing <italic>K. pneumoniae</italic> ST2253 among neonates. Nosocomial surveillance systems are needed to limit the spread of the infection caused by these pathogens resulting from the environmental exposure in NICUs.</p>