AUTHOR=Liu Wei , Xu Yinghua , Dong Derong , Li Huan , Zhao Xiangna , Li Lili , Zhang Ying , Wei Xiao , Wang Xuesong , Huang Simo , Zeng Ming , Huang Liuyu , Zhang Shumin , Yuan Jing 

TITLE=Survey and Rapid Detection of Bordetella pertussis in Clinical Samples Targeting the BP485 in China

JOURNAL=Frontiers in Public Health

VOLUME=3

YEAR=2015

URL=https://www.frontiersin.org/journals/public-health/articles/10.3389/fpubh.2015.00039

DOI=10.3389/fpubh.2015.00039

ISSN=2296-2565

ABSTRACT=<p><italic>Bordetella pertussis</italic> is an important human respiratory pathogen. Here, we describe a loop-mediated isothermal amplification (LAMP) method for the rapid detection of <italic>B. pertussis</italic> in clinical samples based on a visual test. The LAMP assay detected the BP485 target sequence within 60 min with a detection limit of 1.3 pg/μl, a 10-fold increase in sensitivity compared with conventional PCR. All 31 non-pertussis respiratory pathogens tested were negative for LAMP detection, indicating the high specificity of the primers for <italic>B. pertussis</italic>. To evaluate the application of the LAMP assay to clinical diagnosis, of 105 sputum and nasopharyngeal samples collected from the patients with suspected respiratory infections in China, a total of 12 <italic>B. pertussis</italic> isolates were identified from 33 positive samples detected by LAMP-based surveillance targeting BP485. Strikingly, a 4.5 months old baby and her mother were found to be infected with <italic>B. pertussis</italic> at the same time. All isolates belonged to different <italic>B. pertussis</italic> multilocus sequence typing groups with different alleles of the virulence-related genes including four alleles of <italic>ptxA</italic>, six of <italic>prn</italic>, four of <italic>tcfA</italic>, two of <italic>fim2</italic>, and three of <italic>fim3</italic>. The diversity of <italic>B. pertussis</italic> carrying toxin genes in clinical strains indicates a rapid and continuing evolution of <italic>B. pertussis</italic>. This combined with its high prevalence will make it difficult to control. In conclusion, we have developed a visual detection LAMP assay, which could be a useful tool for rapid <italic>B. pertussis</italic> detection, especially in situations where resources are poor and in point-of-care tests.</p>