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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1596811
This article is part of the Research TopicFrom Genomics to Genome Editing: Crop Improvement Innovations for Farmers WorldwideView all 5 articles
Development of Cleaved Amplified Polymorphic Sequence Marker for Powdery Mildew Resistance in Korean Malting Barley Using QTL-seq
Provisionally accepted- 1Winter Crop Research Division, Department of Food Crop Foundation Research and Development, National Institue of Crop Science, Wanju_gun, Republic of Korea
- 2Crop Envrionment Research Division, Depatment of Food Crop Foundation Research and Development, National Institue of Crop Science, Wanju_gun, Republic of Korea
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Introduction: Powdery mildew (PM) caused by Blumeria graminis f.sp. hordei is a major fungal disease affecting barley (Hordeum vulgare L.). The most effective approach to controlling this disease is the development of resistant cultivars. In this study, we investigated the genomic regions associated with PM resistance by performing quantitative loci sequencing (QTL-seq) twice using the parental lines 'Hopum' (susceptible) and 'Jeonju 182' (resistant) as reference genomes.Methods: This study was conducted from 2022 to 2024 at the National Institute of Agricultural Sciences in Wanju, Republic of Korea. We conducted artificial crossing, genomic DNA extraction, phenotypic evaluation, QTL-seq analysis, and cleaved amplified polymorphic sequence (CAPS) marker development. Candidate gene expression was analyzed using real-time quantitative reverse transcription PCR.Results: A total of 2,130 common variants were identified in two regions of chromosome 1H (6,940,595-18,008,713 bp and 19,363,700-20,551,018 bp). Twenty-one non-synonymous single nucleotide polymorphisms among these variants were used to develop CAPS markers, which were validated in an F₂ population and malting barley cultivars. The PMC_75 marker, which is annotated as HORVU.MOREX.r3.1HG0005790, showed a strong association with resistance and was highly expressed in 'Jeonju 182.' This marker is associated with a Clathrin Assembly Protein, which is involved in vesicle formation and intracellular trafficking, processes essential for cellular signaling and defense responses.The development of the CAPS marker (PMC_75) provides a valuable tool for markerassisted selection in breeding PM-resistant malting barley, improving breeding efficiency, and accelerating the development of resistant cultivars.
Keywords: Malting barley, powdery mildew, Resistance, QTL-seq, molecular marker, candidate gene
Received: 20 Mar 2025; Accepted: 09 Apr 2025.
Copyright: © 2025 Park, Yoon, Lee, Hur and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Jin-Cheon Park, Winter Crop Research Division, Department of Food Crop Foundation Research and Development, National Institue of Crop Science, Wanju_gun, Republic of Korea
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