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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Biotechnology
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1572020
CRISPR Mutant Rapid identification in B. napus: RNA-Seq Functional Profiling and Breeding technology Integration
Provisionally accepted- 1 School of Food and Biological Engineering, School of Life Sciences, Jiangsu University, Zhenjiang, China
- 2 School of Life Sciences, Jiangsu University, Zhenjiang, China
Rapeseed breeding through traditional methods is slow and imprecise, while CRISPRbased genome editing emerges as a more potent strategy for crop germplasm enhancement. In this study, we generated the Bnaida mutant by gene editing in the cv ZS11, which is widely used in rapeseed breeding, the phenotypic analysis showed that the petal was attached to the pod and pods were harder to open in edited plant, and then we quickly introduced two Bnaida loci into the elite line of SW1-6 rapidly by backcrossing with edited ZS11 as the donor plant. Locus-specific primer combinations were designed to differentiate heterozygous and homozygous genotypes in backcrossing generations, enabling efficient and rapid selection. This study highlights the integration of gene editing and genotyping selection, offering insights into the future of gene editing-assisted breeding.
Keywords: Brassica napus L., CRISPR/Cas9, IDA-HAE/HSL2 signaling pathway, Rapid identification, RNA-Seq
Received: 06 Feb 2025; Accepted: 27 Mar 2025.
Copyright: © 2025 Rui, Fan, Sarwar, Wang, Dong and Tan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xiao-Li Tan, School of Life Sciences, Jiangsu University, Zhenjiang, China
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