Editorial：Regeneration of Plant Organs in Vitro and Its Mechanistic Basis

Li, Yun; Li, Chenghao; SHAHZAD, ANWAR

doi:10.3389/fpls.2025.1568614

EDITORIAL article

Front. Plant Sci.

Sec. Plant Physiology

Volume 16 - 2025 | doi: 10.3389/fpls.2025.1568614

This article is part of the Research Topic Regeneration of Plant Organs in Vitro and Its Mechanistic Basis View all 5 articles

Editorial：Regeneration of Plant Organs in Vitro and Its Mechanistic Basis

Provisionally accepted

Yun Li ^1*

Chenghao Li ²

ANWAR SHAHZAD ³

¹ Beijing Forestry University, Beijing, China
² Northeast Forestry University, Harbin, Heilongjiang Province, China
³ Aligarh Muslim University, Aligarh, Uttar Pradesh, India

The final, formatted version of the article will be published soon.

There are various factors influencing plant regeneration, with plant growth regulators being among the most critical (Motte et al., 2014). Yan et al. optimized the culture conditions for each developmental stage of Brettschneidera sinensis, they found that the highest seed germination rate was achieved on Murashige and Skoog medium supplemented with 2.0 mg/L 6-benzylaminopurine (6-BA) and 0.2 mg/L 1-naphthaleneacetic acid (NAA). A combination of 1.0 mg/L 6-BA and 0.1 mg/L NAA promoted optimal shoot regeneration, while woody plant medium (WPM) was most effective for adventitious shoot elongation. Additionally, 1/2 MS medium containing 2.0 mg/L indole-3-butyric acid (IBA), 1.0 mg/L NAA, and 20 g/L sucrose resulted in the highest rooting rate. Molecular marker analyses using inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) confirmed the genetic stability of regenerated plants. This study provides an effective technical framework for the conservation and propagation of Brettschneidera sinensis. Brassinolide (BL), in addition to cytokinins and auxins, influences plant regeneration (Jia et

Keywords: plant regeneration, Mechanistic basis, plant growth regulator, Metabolites, Light

Received: 30 Jan 2025; Accepted: 14 Feb 2025.

Copyright: © 2025 Li, Li and SHAHZAD. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Yun Li, Beijing Forestry University, Beijing, China

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