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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1565329
This article is part of the Research Topic Crop Multifunctional Development Based on Utilization of Bioactive Substances or Special Nutrients View all 4 articles
Integrating QTL mapping and GWAS to decipher the genetic mechanisms behind the calcium contents of Brassica napus shoots
Provisionally accepted
Yanan Xiang 1,2 Feng Chen 1 Rui Shi 1 Tinghai Yang 1
Wei Zhang 1 Xiaoying Zhou 1 Chunyun Wang 1
Chengming Sun 1 Sanxiong Fu 1
Xiaodong Wang 1,2
Jiefu Zhang 1,2*
Shen Yue 1*- 1 Jiangsu Academy of Agricultural Sciences (JAAS), Nanjing, China
- 2 School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, China
Brassica napus is an important oil crop worldwide, and its shoots are rich in vitamin C, calcium, and selenium. Functional oilseed-vegetable-dual-purpose varieties can increase the subsidiary value of B. napus. Consumption of high-calcium B. napus shoots can effectively help provide essential elements to the human body. To investigate the genetic mechanisms underlying the calcium concentrations of B. napus shoots, quantitative trait loci (QTL) mapping, using a population of 189 recombinant inbred lines, and a genome-wide association study, using an association panel of 202 diverse accessions, were performed. A total of 12 QTLs controlling calcium content were identified using the recombinant inbred line population in five environments. Among them, qCaC.22GY-A05-1 was considered the major QTL, with a phenotypic variation of 10.10%. In addition, 228 single nucleotide polymorphisms significantly related to calcium content were identified using the genome-wide association study in six environments, and they were distributed on all of the chromosomes, except A10. Finally, 10 candidate genes involved in regulating calcium absorption and transport in B. napus shoots were identified. However, no overlapping intervals were found through a comprehensive analysis of the two datasets. These results provide valuable information for understanding the genetic control of calcium concentration in B. napus shoots.
Keywords: Brassica napus shoots, calcium concentration, QTL mapping, GWAS, candidate genes
Received: 23 Jan 2025; Accepted: 24 Mar 2025.
Copyright: © 2025 Xiang, Chen, Shi, Yang, Zhang, Zhou, Wang, Sun, Fu, Wang, Zhang and Yue. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiefu Zhang, Jiangsu Academy of Agricultural Sciences (JAAS), Nanjing, China
Shen Yue, Jiangsu Academy of Agricultural Sciences (JAAS), Nanjing, China
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