The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 16 - 2025 |
doi: 10.3389/fpls.2025.1553266
This article is part of the Research Topic Secondary Metabolites in Beverage Plant: Metabolism, Function, and Regulation View all 4 articles
Mechanism of endogenous hormones regulating gallic acid biosynthesis during the development of buds and leaves in tea plant (Camellia sinensis)
Provisionally accepted
Yutao Shi 
Xiaofeng Lu Qingying Song Huan Sun Wujing Shen Ruiqi Huang Jiapeng Huang Yanfen Wei Fumin Xiang Xi Wang 
Yanming Tuo 
Jinke Lin *
Yunfei Hu *- Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China
Gallic acid (GA), as a precursor of Epigallocatechin-3-gallate (EGCG) biosynthesis in tea plant, is one of the important components of tea flavor and has various health benefits. However, the mechanism of endogenous hormones regulating GA biosynthesis during the development of buds and leaves of tea shoots is still unclear. In this study, the buds and leaves of five different developmental stages of tea shoots were used as test materials to explore the mechanism of endogenous hormone signaling pathway regulating GA biosynthesis. The results showed that the decrease of D-erythrosyl-4-phosphate content and the increase of shikimic acid content affected the accumulation of GA content during the development of tea shoots. Jasmonic acid, abscisic acid, auxin, cytokinin, and gibberellin inhibited GA biosynthesis by down-regulating the expression of two CsaroDEs through twenty-three plant hormone signal transduction factors, such as CsMYC2, CsSNRK2, CsARR-A, and CsDELLA, respectively, which mediated the downregulation of sixteen transcription factors, such as CsMYB44, CsMYB108, and CsC2C2. CsMYC2 and CsSNRK2 co-mediated the downregulation of the expression of CsMYB44 and CsMYB108 in response to changes in endogenous JA and ABA content, respectively, and inhibited the expression of CsaroDE, thereby co-regulating GA biosynthesis. CsMYC2 may be a key interworking site for the endogenous Jasmonic acid and abscisic acid signaling pathways to jointly regulate GA biosynthesis. Our findings revealed the potential mechanism of endogenous hormones regulating GA biosynthesis during the development of buds and leaves of tea shoots and provided a scientific basis for the regulation of tea quality.
Keywords: Camellia sinensis, Buds and leaves, Development stage, Gallic Acid, biosynthesis, plant hormone signal transduction
Received: 30 Dec 2024; Accepted: 04 Feb 2025.
Copyright: © 2025 Shi, Lu, Song, Sun, Shen, Huang, Huang, Wei, Xiang, Wang, Tuo, Lin and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jinke Lin, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian Province, China
Yunfei Hu, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.