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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Development and EvoDevo
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1538799
This article is part of the Research Topic Apical Meristem Manipulation for Biotechnology Approaches in Plants View all articles
Removing recalcitrance to the micropropagation of five farmerpreferred cassava varieties in Côte d'Ivoire by supplementing culture medium with kinetin or thidiazuron
Provisionally accepted
SEKA SEKA JOHN STEVEN 1*
Kan Modeste KOUASSI 1
Edwige F. YEO 1
Flavie M. SAKI 1
Daniel Houa OTRON 1
Fidele Tiendrebeogo 2* Angela O. ENI 1
Nazaire Koffi II KOUASSI 1 Justin S. PITA 1- 1 Central and West African Virus Epidemiology (WAVE), Bingerville, Côte d'Ivoire
- 2 Institut de l'environnement et de la Recherche Agricole (INERA), Ouagadougou, Burkina Faso
In vitro micropropagation is a rapid method of multiplying healthy planting material to control Cassava mosaic disease (CMD), one of a major constraint to cassava production in Africa. However, some cassava varieties have a low propagation ratio under in vitro conditions. The main objective of this study was to improve the in vitro propagation rate of five difficult to grow, farmer-preferred cassava varieties using plant growth regulators. Microcuttings from in vitro plantlets of five recalcitrant cassava varieties (Agbablé 3, Ampong, Bayérè, Bocou 5, Olékanga) were evaluated for their capacity to rapidly regenerate plantlets. Time to root or leaf formation, number of nodes, number of roots, and the in vitro plantlet length were evaluated on nine culture media combinations. We found among all the cassava varieties studied, the shortest times for leaf (4 to 7 days) or root (9 to 14 days) formation were recorded when the two types of MS media were supplemented with kinetin and thidiazuron as well as on the medium contain half-strength MS without these plant growth regulators. These two hormones evaluated were better for regeneration of leaves, nodes and elongation of in vitro plantlets with optimum concentration of 5 and 10 nM or thidiazuron, and 0.12 or 0.24 µM for KIN. A survival rate between 85-91% was recorded under tunnel conditions and the plantlets appeared to be morphologically normal. The information obtained during this study will be useful for mass multiplication programs of elite cassava varieties.
Keywords: In vitro Propagation, recalcitrant cassava varieties, Thidiazuron, Kinetin, Virus-free plantlets
Received: 03 Dec 2024; Accepted: 18 Mar 2025.
Copyright: © 2025 SEKA JOHN STEVEN, KOUASSI, YEO, SAKI, OTRON, Tiendrebeogo, ENI, KOUASSI and PITA. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
SEKA SEKA JOHN STEVEN, Central and West African Virus Epidemiology (WAVE), Bingerville, Côte d'Ivoire
Fidele Tiendrebeogo, Institut de l'environnement et de la Recherche Agricole (INERA), Ouagadougou, Burkina Faso
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