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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 16 - 2025 |
doi: 10.3389/fpls.2025.1538607
This article is part of the Research Topic Plant Specialized Metabolisms: Physiological Responses and Molecular Mechanisms View all 10 articles
CaMYBA-CaMYC-CaTTG1 complex activates the transcription of anthocyanin synthesis structural genes and regulates anthocyanin accumulation in pepper (Capsicum annuum L.) leaves
Provisionally accepted- Zhejiang Agriculture and Forestry University, Hangzhou, China
Anthocyanins are flavonoid-derived metabolites that contribute to plant and human health. At present, few studies have studied the biosynthesis and accumulation mechanism of anthocyanins in pepper leaves. The role of CaMYBA-CaMYC-CaTTG1 complex in anthocyanin biosynthesis in pepper leaves was studied. Yeast two-hybrid and double-luciflucifase experiments showed that CaMYBA, CaMYC and CaTTG1 could form a MBW complex. It also has transcriptional activation on the anthocyanin synthesis structural genes of CaCHS, CaCHI, CaF3H, CaF3'5'H, CaANS, CaDFR, CaUFGT. Silencing CaMYBA or CaMYC could decrease the content of anthocyanin in pepper leaves. Transient overexpression of CaMYBA in tobacco indicated that CaMYBA determines the function of MBW complex. Further analysis showed that CaMYBA could activate the expression of CaMYC by binding to its promoter. Overall, our study expands the understanding of the regulatory mechanism of anthocyanin synthesis in pepper leaves, and has important significance for creating more pepper plants with different color patterns by gene editing engineering.
Keywords: pepper, Anthocyanin, MBW complex, regulatory complex, Transcriptional regulation
Received: 03 Dec 2024; Accepted: 04 Feb 2025.
Copyright: © 2025 Ma, Liang, Xu, Lin and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Biao Zhu, Zhejiang Agriculture and Forestry University, Hangzhou, China
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