Accumulation Differences of High-value Ingredients in Different Phenotype Lonicera macranthoides: Insights from Integrative Metabolome and Transcriptome Analyses

Juan Zeng Yuqing Long ^* Jiayuan Zhu Xuesen Fu ^* Jingyu Zhang ^* Jiawei He ^* Xiaorong Liu ^* Zhihui Wang ^* Qiao-zhen Tong Xiangdan Liu ^* Ribao Bao Zhou ^*

• Hunan University of Chinese Medicine, Changsha, China

Hand.-Mazz., the primary sources of Lonicerae Flos(Shanyinhua), brings great medicinal and economic value as an invaluable source of natural bioactive compounds. Nutrient and metabolites accumulation generally changed accompany with its floral development and opening. While the specific accumulation pattern and the underlying molecular regulatory networks remain unclear. The present study intergrated a comparative analysis upon UPLC-MS/MS-based metabolomics and RNA-seq-based transcriptomics to revealed a significant differences in accumulation of flavonoids, phenolic acids, and terpenoids during the late stage of flower development between the xianglei-type (corolla-closed) and wild-type (corolla-unfolded) of L. macranthoides flowers. Combined analysis further identified 36 hub genes, major transcription factors and hormonerelated genes, which play key roles in the differential accumulation of the abovementioned metabolites. These lines of evidences provide a molecular basis for the metabolic changes occurring during growth and can be significantly implicated in further research on the biosynthetic pathways associated with high-value potent active components in woody plants. immunity, reducing blood levels of sugar and lipids, and anti-atherosclerosis effect (Commission,2005;