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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1527635

Genome-wide association for multiple quantitative traits in forage oat germplasm based on Specific Length Amplified Fragment Sequencing (SLAFseq)

Provisionally accepted
Yue Li Yue Li 1Kai Zhu Kai Zhu 2Huiting Cui Huiting Cui 3Qiannan Hu Qiannan Hu 1Chu Wang Chu Wang 1Fang Jia Fang Jia 4Junmei Kang Junmei Kang 2Mingna Li Mingna Li 2Chengze Ma Chengze Ma 1Yan Sun Yan Sun 1*
  • 1 China Agricultural University, Beijing, Beijing Municipality, China
  • 2 Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
  • 3 Henan Institute of Science and Technology, Xinxiang, Henan Province, China
  • 4 Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry, Beijing, China

The final, formatted version of the article will be published soon.

    Oats (Avena sativa L.) is an ideal forage species due to its excellent production performance, high nutritional value and robust adaptability. In the present study, we analyzed plant height (PH), panicle length (PL), stem diameter (SD), node number (NN), flag leaf length (FLL), flag leaf width (FLW), second leaf length (SLL) and second leaf width (SLW) in a collection containing 340 forage oat accessions, in order to perform genome-wide association study (GWAS) for identifying markers associated to the 8 traits. We genotyped this collection using Specific Length Amplified Fragment Sequencing (SLAF-seq).Phenotypes for PH, PL, SD, NN, FLL, FLW, SLL and SLW were collected under natural condition in 4 environments. GWAS analyses detected six significant associations for NN and 3 for FLL. Candidate genes of the 9 associations were screened and discussed. Several genes were found to be associated with node number, including zinc finger MYM-type protein 1-like isoform X1, ervatamin-B-like, Pimeloyl-ACP methyl ester carboxylesterase, ACT domain-containing protein ACR4-like involved in cell division and organ development.Additionally, three genes were linked to flag leaf length: putative aquaporin PIP2-2, triacylglycerol lipase OBL1-like, and scarecrow-like protein 21 involved in regulation of plant development and stress response. The use of these SNP markers may be useful to accelerate the breeding progress of forage oat in temperate monsoon environments.

    Keywords: Avena sativa L., Genotyping by sequencing (GBS), Node number, Flag leaf length, Genome wide association analysis (GWAS)

    Received: 13 Nov 2024; Accepted: 20 Jan 2025.

    Copyright: © 2025 Li, Zhu, Cui, Hu, Wang, Jia, Kang, Li, Ma and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yan Sun, China Agricultural University, Beijing, 100083, Beijing Municipality, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.