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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Breeding
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1524912
Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS
Provisionally accepted
Alma Kokhmetova 1*
Nagenahalli Dharmegowda Rathan 2*
Deepmala Sehgal 3*
Yuliya Zeleneva 4
Madina Kumarbayeva 1
Ardak Bolatbekova 1
Gopalareddy Krishnappa 5* Zhenis Keishilov 1 Asia Kokhmetova 1 Kanat Mukhametzhanov 1 Kanat Bakhytuly 1- 1 Institute of Plant Biology and Biotechnology (IPBB), Almaty, Kazakhstan
- 2 Coverta Agriscience, Hyderabad, Telangana, India., Hyderabad, India
- 3 Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire, United Kingdom., Berkshire, United Kingdom
- 4 Laboratory of Mycology and Phytopathology, All Russian Institute of Plant Protection., St. Petersburg-Pushkin, Russia
- 5 Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, Coimbatore, Kerala, India
For identifying genomic regions associated with resistance to Septoria tritici blotch (STB) and Septoria nodorum blotch (SNB) in wheat, a genome-wide association study (GWAS) was conducted using a diverse panel of 191 spring and winter wheat genotypes. The panel was genotyped using DArTseq TM technology and phenotyped under natural field conditions for three cropping seasons (2019-2020, 2020-2021, and 2021-2022) and under artificially inoculated field conditions for two cropping seasons (2020-2021 and 2021-2022). Additionally, the panel was phenotyped under greenhouse condition for five mixed isolates of Zymoseptoria triticiSTB (five mixed isolates in a single experiment) and SNB, (four independent isolates, and a purified toxin in five different independent experiments) of Parastagonospora nodorum under greenhouse conditions. GWAS identified nine marker -trait associations (MTAs), including six MTAs for different isolates under greenhouse conditions, two MTAs under natural field conditions, and one MTA under artificially inoculated field conditions. A pleiotropic SNP MTA (100023665) was identified on chromosome 5B governing resistance against SNB isolate Pn Sn2K_USA and SNB purified toxin Pn ToxA_USA and, explaining 30.73% and 46.94% of phenotypic variation, respectively. In silico analysis identified important candidates genes belonging to the leucine-rich repeat (LRR) domain superfamily, Zinc finger GRF-type transcription factors, potassium transporters, nucleotide-binding site (NBS) domain superfamily, disease resistance protein, P-loop containing nucleoside triphosphate hydrolase, virus X resistance proteinApoptotic proteaseactivating factors, and NB-ARC domains.
Keywords: MTAS, Septoria tritici blotch, Septoria nodorum blotch, GWAS, candidate genes
Received: 08 Nov 2024; Accepted: 07 Apr 2025.
Copyright: © 2025 Kokhmetova, Rathan, Sehgal, Zeleneva, Kumarbayeva, Bolatbekova, Krishnappa, Keishilov, Kokhmetova, Mukhametzhanov and Bakhytuly. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Alma Kokhmetova, Institute of Plant Biology and Biotechnology (IPBB), Almaty, Kazakhstan
Nagenahalli Dharmegowda Rathan, Coverta Agriscience, Hyderabad, Telangana, India., Hyderabad, India
Deepmala Sehgal, Syngenta, Jealott's Hill International Research Centre, Bracknell, Berkshire, United Kingdom., Berkshire, United Kingdom
Gopalareddy Krishnappa, Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, Coimbatore, 641007, Kerala, India
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