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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Biotechnology
Volume 16 - 2025 |
doi: 10.3389/fpls.2025.1516963
This article is part of the Research Topic Plant-Based Solutions for Sustainable Agriculture and Environmental Remediation View all articles
Exploring the Potential Role of EPSPS Mutations for Enhanced Glyphosate Resistance in Nicotiana tabacum
Provisionally accepted- Chinese Academy of Agricultural Sciences (CAAS), Beijing, Beijing Municipality, China
Glyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, the emergence of glyphosate-resistant weeds has driven the need for enhanced herbicide resistance in crops. In this study, we engineered two mutant variants of the tobacco EPSPS gene through amino acid substitution (TIPS-NtEPSPS and P180S-NtEPSPS). These mutated EPSPS genes were overexpressed in tobacco under the control of CaMV35S promoters. Our results demonstrate that overexpression of TIPS-NtEPSPS significantly enhances glyphosate tolerance, allowing plants to withstand up to four times the recommended dose without compromising their fitness. This research highlights the potential of the TIPS-NtEPSPS mutant to improve herbicide resistance in tobacco, offering a viable approach for effective weed management.
Keywords: glyphosate, Herbicide Resistance, EPSP synthase, Tobacco, gene editing
Received: 25 Oct 2024; Accepted: 16 Jan 2025.
Copyright: © 2025 Li, Chen, Cui, Sun, Jing and Dai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Lv Jing, Chinese Academy of Agricultural Sciences (CAAS), Beijing, 100081, Beijing Municipality, China
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