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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1495779

Optimization of Extraction and Enrichment Process of Cannabidiol from Industrial Hemp and Evaluation of Its Bioactivity

Provisionally accepted
Junkai Wu Junkai Wu 1Xiaomeng Zhang Xiaomeng Zhang 2Xiaoqing Liu Xiaoqing Liu 2Zunlai Sheng Zunlai Sheng 2*Jianping Hu Jianping Hu 1Feiyan Zhang Feiyan Zhang 1
  • 1 Quanzhou Medical College, Quanzhou, China
  • 2 Northeast Agricultural University, Harbin, Heilongjiang Province, China

The final, formatted version of the article will be published soon.

    The Cannabis Sativa L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential. The extraction parameters for CBD from industrial hemp were optimized using the Box-Behnken design and response surface methodology (RSM). The purification process involved characterizing the penetration and desorption profiles of CBD on HPD-100 resin. The in vitro antibacterial activity was assessed by determining the minimum inhibitory concentration (MIC) against Staphylococcus aureus and Escherichia coli. Antioxidant properties were evaluated using DPPH and ABTS assays, as well as an iron-reducing ability test.After optimization, the extraction rate of CBD reached 0.26 ± 0.02%. The use of HP-100 resin in the purification process resulted in a significant enrichment of CBD content, which was 4.2 times higher than that of the crude extract, with a recovery rate of 83.13%. The MIC against S. aureus was found to be 5 mg/mL, while no inhibitory effect was observed against E. coli. The IC50 values for the DPPH and ABTS assays were 0.1875 mg/mL and 2.988 mg/mL, respectively, indicating the potent antioxidant capacity of CBD. Additionally, CBD demonstrated a strong ironreducing ability. These findings contribute to the development of CBD for broader applications in various industries, highlighting its potential as a valuable compound in health and wellness sectors.

    Keywords: Industrial hemp, Cannabidiol, Extraction process, enrichment process, bioactivity evaluation

    Received: 13 Sep 2024; Accepted: 13 Jan 2025.

    Copyright: © 2025 Wu, Zhang, Liu, Sheng, Hu and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Zunlai Sheng, Northeast Agricultural University, Harbin, 150030, Heilongjiang Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.