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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Genetics, Epigenetics and Chromosome Biology
Volume 16 - 2025 |
doi: 10.3389/fpls.2025.1494694
Revealing that artificial reproduction promotes increased genetic diversity between generations in Carpinus putoensis
Provisionally accepted
Dingsheng Li 1*
Kai Gao 2 Yeping Chen 3* Haojie Gao 3* Haiming Huang 1* Bo Ye 3* Lei Shi 3* Haina Yu 1* Ying Zhao 3*- 1 Zhoushan Forest Farm of Zhejiang, Zhoushan, China
- 2 Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China
- 3 Zhoushan Academy of Forestry, Zhoushan, China
Carpinus putoensis, an endemic species of Putuo Island in the Zhoushan Archipelago, Zhejiang Province, China, is listed as critically endangered (D1) in the 1998 World List of Threatened Trees. Using 15 pairs of SSR primers markers, 143 individuals from three population generations were analyzed, generating 193 alleles. The average number of alleles (Na) was 12.9, ranging from 4 to 28, while the average effective number of alleles (Ne) was 4.900, with a range of 1.649 to 11.602.The multilocus outcrossing rate (tm) was 1.000, and the single-locus outcrossing rate (ts) was 0.871, ranging from 0.751 to 0.920 across the families studied. The difference between tm and ts (0.129) and the positive biparental inbreeding coefficients (0.080 to 0.249) indicate the presence of inbreeding. Moreover, an increase in Na and Ne was observed across generations, from 6.400 and 2.838 in the F1 generation to 9.200 and 4.228 in the F3 generation, respectively. These results highlight the need for artificial interventions to increase population size and improve genetic diversity, which are critical factors for the conservation and recovery of C. putoensis.
Keywords: Carpinus putoensis, genetic diversity, mating system, SSR, endangered plants
Received: 09 Oct 2024; Accepted: 07 Feb 2025.
Copyright: © 2025 Li, Gao, Chen, Gao, Huang, Ye, Shi, Yu and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Dingsheng Li, Zhoushan Forest Farm of Zhejiang, Zhoushan, China
Yeping Chen, Zhoushan Academy of Forestry, Zhoushan, China
Haojie Gao, Zhoushan Academy of Forestry, Zhoushan, China
Haiming Huang, Zhoushan Forest Farm of Zhejiang, Zhoushan, China
Bo Ye, Zhoushan Academy of Forestry, Zhoushan, China
Lei Shi, Zhoushan Academy of Forestry, Zhoushan, China
Haina Yu, Zhoushan Forest Farm of Zhejiang, Zhoushan, China
Ying Zhao, Zhoushan Academy of Forestry, Zhoushan, China
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