Breeding in winter wheat (Triticum aestivum L.) can be further progressed by targeting previously neglected competitive traits

Annette Manntschke ¹ Lina Hempel ¹ Andries Temme ^1,2 Marcin Reumann ¹ Tsu-Wei Chen ^1*

• ¹ Institute of Horticultural Production Systems, Albrecht Daniel Thaer Institute for Agricultural and Horticultural Sciences, Faculty of Life Sciences, Humboldt University of Berlin, Berlin, Germany
• ² Laboratory of Plant Breeding, Wageningen University and Research, Wageningen, Netherlands, Netherlands

Breeders work to adapt winter wheat genotypes for high planting densities to pursue sustainable intensification and maximize canopy productivity. Although the effects of plant-plant competition at high planting density have been extensively reported, the quantitative relationship between competitiveness and plant performance remains unclear. In this study, we introduced a shoot competitiveness index (SCI) to quantify the competitiveness of genotypes and examined the dynamics of nine competitiveness-related traits in 200 winter wheat genotypes grown in heterogeneous canopies at two planting densities. Higher planting densities increased plant shoot length but reduced biomass, tiller numbers, and leaf mass per area (LMA), with trait plasticity showing at least 41% variation between genotypes. Surprisingly, genotypes with higher LMA at low density exhibited greater decreases under high density, challenging expectations from game theory. Regression analysis identified tiller number, LMA, and plant shoot length as key traits influencing performance under high density. Contrary to our hypothesis, early competitiveness did not guarantee sustained performance, revealing the dynamic nature of plant-plant competition. Our evaluation of breeding progress across the panel revealed a declining trend in SCI (R² = 0.61), aligning with the breeding objective of reducing plant height to reduce individual competitiveness and increase the plant-plant cooperation. The absence of historical trends in functional traits and their plasticities, such as tiller number and LMA, suggests their potential for designing ideal trait-plasticity for plant-plant cooperation and further crop improvement.