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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Pathogen Interactions
Volume 16 - 2025 | doi: 10.3389/fpls.2025.1485160
This article is part of the Research Topic Improving Legume Crops for Resistance to Pathogens and Pests View all 3 articles

Nematicidal potential of Microbacterium maritypicum Sneb159 against Heterodera glycines and the complete genome sequence analysis

Provisionally accepted
Jing Zhao Jing Zhao 1,2Zhifu Xing Zhifu Xing 3Aatika Sikandar Aatika Sikandar 4Yuxi Duan Yuxi Duan 2*
  • 1 Hainan University, Haikou, Hainan Province, China
  • 2 Shenyang Agricultural University, Shenyang, China
  • 3 Guizhou University, Guiyang, Guizhou Province, China
  • 4 Guangxi University, Nanning, Guangxi Zhuang Region, China

The final, formatted version of the article will be published soon.

    Heterodera glycines is one of the most important pathogens of soybean production worldwide. The present study demonstrated that M. maritypicum Sneb159 fermentation broth showed strong nematicidal activities against H. glycines. The filtrate rather than bacterial cells played a role in the nematicidal character, and reduced the invasion number as well as suppressed the development of juveniles in soybean. The analysis of chemotaxis showed that M. maritypicum Sneb159 has a repellent effect on H. glycines in pot experiments. The volatiles produced by M. maritypicum Sneb159 are toxic to H. glycines for both juveniles and eggs. The seven compounds were analyzed using solidphase microextraction gas chromatography-mass spectrometry (SPME-GC-MS). In the bioassays, dimethyl disulfide and dimethyl trisulfide showed both direct contact and fumigated effect on juveniles and eggs. The complete genome sequence of the bacterium M. maritypicum Sneb159 was completed using the PacBio sequencing platform. The genome comprised 3895529 bp and a 68.63% G + C content. Three secondary metabolites gene clusters were predicted by the antiSMASH system. The findings reveal multifunction of M. maritypicum Sneb159 towards H. glycines, and has the potential to be developed as a safe nematicidal agent.

    Keywords: Heterodera glycines, Microbacterium maritypicum, Nematicidal activity, Volatile organic compound, complete genome

    Received: 23 Aug 2024; Accepted: 13 Jan 2025.

    Copyright: © 2025 Zhao, Xing, Sikandar and Duan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yuxi Duan, Shenyang Agricultural University, Shenyang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.