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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 16 - 2025 |
doi: 10.3389/fpls.2025.1476810
E-Nose and HS-SPME-GC-MS Unveiling the Scent Signature of Ligusticum chuanxiong and Its Medicinal Relatives
Provisionally accepted- 1 Sichuan Agricultural University, Ya'an, China
- 2 Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan Province, China
To explore the affinities between Ligusticum chuanxiong and its medicinal relatives, we investigated the species origin and evolution of cultivation of Ligusticum chuanxiong. This study encompassed seven species from various origins, including Chuanxiong (Ligusticum chuanxiong Hort.), Gansu Chuanxiong (Ligusticum chuanxiong cv. Gansu), Yunnan Chuanxiong (Ligusticum chuanxiong cv. Yunnan), Japanese Chuanxiong (Cnidium officinale Makino), Fuxiong (Ligusticum sinense 'Fuxiong'), Gaoben (Ligusticum sinense), and Liaogaoben (Ligusticum jeholense), comprising 27 distinct materials. We employed headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) to identify various odor profiles from these species using electronic nose technology (E-nose). The method effectively identified volatile constituents in the leaves of these seven species. Results indicated that odor differences between L. chuanxiong and its medicinal relatives were predominantly observed in sensors W1W and W1S. Linear discriminant factor analysis (LDA) successfully distinguished five of the relatives; however, L. chuanxiong and L. sinense exhibited high odor similarity, limiting complete differentiation in some samples. HS-SPME-GC-MS identified a total of 118 volatile constituents, with eight differential volatiles identified: trans-Neocnidilide, β-Caryophyllene, β-Selinene, 5-Pentylcyclohexa-1,3-diene , (E)-Ligustilide, Butylphthalide, Neophytadiene, and Senkyunolide. Hierarchical cluster analysis (HCA) grouped L. chuanxiong, L. sinense, L. jeholense, and L. chuanxiong cv. Gansu together, highlighting the close relationship between L. chuanxiong and L. sinense. Joint analysis revealed a significant positive correlation between sensor W1W and the differential volatile component β-Caryophyllene, suggesting its potential for distinguishing closely related species. This study provides a foundational understanding of volatile components in the leaves of L. chuanxiong and its medicinal relatives using E-nose combined with HS-SPME-GC-MS, contributing to the discussion on their interspecific odor characteristics and relationships.
Keywords: Ligusticum chuanxiong Hort., E-nose, Headspace solid phase microextraction, Gas Chromatography-Mass Spectrometry, Volatile components
Received: 19 Aug 2024; Accepted: 14 Jan 2025.
Copyright: © 2025 Wanjing, Fang and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Xu Wanjing, Sichuan Agricultural University, Ya'an, China
Peng Fang, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan Province, China
Zhengjun Xu, Sichuan Agricultural University, Ya'an, China
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