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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Crop and Product Physiology
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1527212
This article is part of the Research Topic Elucidating the Molecular, Physiological, and Biochemical Mechanisms Underlying Stress Responses in Crop Plants View all 3 articles
LC-MS/MS-based metabolomic study provides insights into altitudedependent variations in flavonoid profiles of strawberries
Provisionally accepted
Muhammad Junaid Rao
1,2
Huaizheng Wang
2
Huaming Lei
2*
Hongcha Zhang
2*
显德 段
2
Liuyuan Bao
2*
Yang Chengcui
2
Duo Han
2*
Yongzhi Zhang
2*
Shunqiang Yang
2*
Mingzheng Duan
2*- 1 School of Forestry and Biotechnology, Zhejiang Agriculture & Forestry University, Hangzhou, China
- 2 Zhaotong University, Zhaotong, Yunnan, China
Environmental conditions significantly influence the metabolic composition and quality attributes of fruits. This study investigated the impact of altitude-associated environmental variation on flavonoid profiles and fruit quality parameters by comparing the "Red Face" strawberry variety grown in two distinct locations: high-altitude-associated environmental conditions in Zhaotong and low-altitude conditions in Dandong. Using LC-MS/MS analysis, we identified 163 bioactive flavonoids, comprising 85 flavonols, 37 flavanones, 33 flavones, and 8 flavanonols. The high-altitude environment of Zhaotong significantly enhanced specific flavonoid compounds, with notable increases in neohesperidin (20.4-fold), tamarixetin-3-O-glucoside-7-O-rhamnoside (17.7-fold), isovitexin (9.1-fold), and hesperidin (8.5-fold) compared to Dandong-grown fruits. Conversely, Dandong-grown fruits showed higher levels of chrysoeriol-7-O-glucoside (53.9fold), 6-hydroxykaempferol-6,7-O-diglucoside (36.3-fold), and eucalyptin (9.7-fold). The tricetin 3'-glucuronide (24.49% vs 15.31%) and quercetin-4'-O-glucuronide (24.15% vs 15.59%), are the major flavonoids identified in Zhaotong strawberries than Dandong-grown fruits. Furthermore, strawberries cultivated in Zhaotong demonstrated superior antioxidant activities and capacity, increased quality parameters, including higher sugar content (15.30 °Brix vs 10.96 °Brix), increased ascorbic acid (15.73 mg/g vs 8.53 mg/g), and optimal firmness (20.51 N vs 23.16 N) than Dandong strawberries. These findings suggest that high-altitude cultivation conditions positively influence strawberry fruit characteristics, enhancing both bioactive compound profiles and overall fruit quality. This research provides valuable insights for optimizing strawberry cultivation conditions to maximize nutritional and commercial value.
Keywords: Strawberry fruit, Bioactive flavonoids, fruit quality, Antioxidant activities, Environment conditions
Received: 13 Nov 2024; Accepted: 11 Dec 2024.
Copyright: © 2024 Rao, Wang, Lei, Zhang, 段, Bao, Chengcui, Han, Zhang, Yang and Duan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Huaming Lei, Zhaotong University, Zhaotong, 657000, Yunnan, China
Hongcha Zhang, Zhaotong University, Zhaotong, 657000, Yunnan, China
Liuyuan Bao, Zhaotong University, Zhaotong, 657000, Yunnan, China
Duo Han, Zhaotong University, Zhaotong, 657000, Yunnan, China
Yongzhi Zhang, Zhaotong University, Zhaotong, 657000, Yunnan, China
Shunqiang Yang, Zhaotong University, Zhaotong, 657000, Yunnan, China
Mingzheng Duan, Zhaotong University, Zhaotong, 657000, Yunnan, China
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