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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Nutrition
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1522481
This article is part of the Research Topic Interplay Between Plant Nutrient Uptake and Abiotic Stress View all articles

Dihydroporphyrin iron (III) enhances low temperature tolerance by increasing carbon and nitrogen metabolism in Andrographis paniculata

Provisionally accepted
Jian Shao Fen Jian Shao Fen 1Huang Yan Fen Huang Yan Fen 1Chen Dong Liang Chen Dong Liang 2Chu Zhong Chu Zhong 1*
  • 1 Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Medicinal Botanical Garden, Nanning, China
  • 2 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi Zhuang Region, China

The final, formatted version of the article will be published soon.

    Dihydroporphyrin iron (DH-Fe) is a novel plant growth regulator that plays signifi-cant roles in plant stress resistance. We found that Andrographis paniculata is extremely sensitive to low temperature (LT) with a threshold of 25 °C. To evaluate whether and how DH-Fe alleviates LT stress in A. paniculata, different DH-Fe concentrations (0, 10, 20, and 40 μg·L-1) were applied to estimate its effects on C and N metabolism and antioxidative capacity in A. paniculata grown under 20 °C. Pre-treatment of DH-Fe alleviated LT-induced anthocyanin accumulation. Additionally, it relieved LT-induced oxidative stress by increasing the activity of catalase (CAT). DH-Fe reduced the con-tents of sucrose, soluble sugar and starch and the activities of sucrose synthase (SS) and hexokinase (HXK), but stimulated the activities of sucrose phosphate synthase (SPS), glucose-6-phosphate dehydrogenase (G6PDH), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), phosphoenolpyruvate carboxylase (PEPC), isocitrate de-hydrogenase (ICDH), and malic enzyme (ME). Soluble protein and proline contents were decreased by DH-Fe, while total N and free amino acids contents were increased, accompanying by the enhancement of the activities of glutamine synthase (GS), glu-tamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GTP). Simultaneously, the content of andrographolide, the bioactive ingredient of A. panic-ulata, was remarkably declined. These results indicated that DH-Fe alleviates LT-induced oxidation by increasing sugar catabolism and allocating C metabolic flow to N assimilation. A concentration of 20 μg·L-1 DH-Fe is recommended to be used to enhance LT tolerance in A. paniculata. Our results update the understanding of the mechanism of plant cold tolerance and provide new ideas for relieving plant cold damage.

    Keywords: dihydroporphyrin iron, low temperature stress, Carbon Metabolism, Andrographis paniculate, antioxidant

    Received: 04 Nov 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 Fen, Fen, Liang and Zhong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Chu Zhong, Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Medicinal Botanical Garden, Nanning, China

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