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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Plant Abiotic Stress
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1521758
This article is part of the Research Topic Integrative Multi-omics and Artificial Intelligence (AI)-driven Approaches for Superior Nutritional Quality and Stress Resilience in Crops View all articles
Identification of methyltransferase and demethylase genes and their expression profiling under biotic and abiotic stress in pigeon pea (Cajanus cajan [L.] Millspaugh)
Provisionally accepted
Priyanka Kumari
1,2
Sougata Bhattacharjee
1,2
Venkat Raman K.
2
Jyotsana Tilgam
1,2
Krishnayan Paul
1,2
Kameshwaran Senthil
2
Mahi Baaniya
1,2
Rama Prashat G
1
Rohini Sreevathsa
2
Debasis Pattanayak
2*- 1 Indian Agricultural Research Institute (ICAR), New Delhi, National Capital Territory of Delhi, India
- 2 National Institute for Plant Biotechnology, Indian Council of Agricultural Research, New Delhi, India
The methylation-demethylation dynamics of RNA plays major roles in different biological functions, including stress responses, in plants. m 6 A methylation in RNA is orchestrated by a coordinated function of methyl transferases (writers) and demethylases (Erasers). Genome-wide analysis of genes involved in methylation and demethylation was performed in pigeonpea. Blast search, using Arabidopsis gene sequences, resulted in the identification of two methylation genes (CcMTA70, CcMTB70), two genes encoding adaptor proteins for methylation (CcFIPA and CcFIPB) and 10 demethylase (ALKBH) genes (CcALKBH1A, CcALKBH1B, CcALKBH1C, CcALKBH2, CcALKBH8, CcALKBH8A, CcALKBH8B, CcALKBH9, CcALKBH10A and CcALKBH10B) in the pigeonpea genome. The identified genes were analyzed through phylogenetic relationship, chromosomal position, gene structure, conserved motif, domain and subcellular location prediction etc. These structural analyses resulted in categorization of MTs and FIPs into one group, i.e., CcMTA/B and CcFIPA/B, respectively; and ALKBHs into four groups, viz. CcALKBH1/2, CcALKBH8, CcALKBH9 and CcALKBH10. Relative expression analysis of the identified genes in various tissues at different developmental stages revealed the highest level of expression in leaf and the least in root. CcMTs and CcFIPs had similar patterns of expression, and CcALKBH10B demonstrated the highest and CcALKBH2 the lowest level of expression in all the tissues analyzed. CcALKBH8 showed the highest induction in expression upon exposure to heat stress, and CcALKBH10B demonstrated the highest level of induction in expression during drought, salt and biotic (Helicoverpa armigera infestation) stresses. The present study would pave the way for detailed molecular characterization of m 6 A methylation in pigeonpea and its involvement in stress regulation.
Keywords: Methylation, Demethylation, pigeon pea, M 6 A modification, ALKBHs
Received: 05 Nov 2024; Accepted: 18 Dec 2024.
Copyright: © 2024 Kumari, Bhattacharjee, K., Tilgam, Paul, Senthil, Baaniya, Prashat G, Sreevathsa and Pattanayak. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Debasis Pattanayak, National Institute for Plant Biotechnology, Indian Council of Agricultural Research, New Delhi, India
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