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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1518962
This article is part of the Research Topic Research on Brassicaceae Crops Genomics and Breeding, Volume II View all 8 articles

Chinese cabbage orphan gene BR3 confers bolting resistance to Arabidopsis through the gibberellin pathway

Provisionally accepted
Yuting Zhang Yuting Zhang 1Mingliang Jiang Mingliang Jiang 2Shurui Sun Shurui Sun 1Zongxiang Zhan Zongxiang Zhan 1Xiaonan Li Xiaonan Li 1*Zhongyun Piao Zhongyun Piao 1*
  • 1 Shenyang Agricultural University, Shenyang, China
  • 2 Jilin Agricultural Science and Technology University, Jilin, China

The final, formatted version of the article will be published soon.

    Premature bolting reduces the yield and quality of Chinese cabbage, making bolting resistance gene identification crucial for breeding superior and stable varieties. In this study, we identified an orphan gene BOLTING RESISTANCE 3 (BR3) that positively regulates bolting resistance in Arabidopsis thaliana. The expression of BR3 was developmentally regulated and occurred during the seedling and flowering stages. The BR3 protein was localized to both the plasma membrane and nucleus. Arabidopsis BR3 overexpressing (BR3OE) plants exhibited delayed bolting and flowering times, an increased number of rosette leaves, reduced plant height, and fewer siliques under long-day (LD) conditions. Key flowering genes were significantly downregulated in BR3OE plants. BR3OE plants similarly exhibited delayed bolting and flowering times, and an increased number of rosette leaves under short-day (SD) conditions. BR3OE plants showed no significant phenotypic differences after vernalization treatment. BR3OE and WT plants exhibited early flowering after GA3 treatment, and bolting and flowering time remained delayed in BR3OE plants compared with WT plants. Key DELLA genes BrRGA1 and BrRGL3 exhibited a co-expression pattern consistent with BR3 gene in Chinese cabbage, which suggested that BrRGA1 and BrRGL3 genes may directly or indirectly regulated by BR3 gene. BR3 gene increased bolting resistance perhaps by upregulating the expression of DELLA genes in the GA pathway. This study provides new theoretical insights for addressing premature bolting in Chinese cabbage and offers novel approaches for breeding bolting-resistant varieties.

    Keywords: Chinese cabbage, orphan gene, BR3, Bolting resistance, Arabidopsis, GA pathway

    Received: 29 Oct 2024; Accepted: 26 Dec 2024.

    Copyright: © 2024 Zhang, Jiang, Sun, Zhan, Li and Piao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xiaonan Li, Shenyang Agricultural University, Shenyang, China
    Zhongyun Piao, Shenyang Agricultural University, Shenyang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.