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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Metabolism and Chemodiversity
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1513875
This article is part of the Research Topic Bioactive Compounds, Functional Ingredients, Antioxidants, and Health Benefits of Edible Plants Volume II View all 4 articles

Simultaneous Quantitative Screening of 53 Phytochemicals from Rheum tataricum L. Roots: A Comparative Study of Supercritical CO2, Subcritical Ethanol, and Ultrasound-Assisted Extraction for Enhanced Antioxidant, Antibacterial Activities, and Molecular Docking Study

Provisionally accepted
Madina Amangeldinova Madina Amangeldinova 1Mehmet Ersatır Mehmet Ersatır 2adem necip adem necip 3Mustafa Abdullah YILMAZ Mustafa Abdullah YILMAZ 4Mehmet Cimentepe Mehmet Cimentepe 5Nataliya Kudrina Nataliya Kudrina 1*Nina V. Terletskaya Nina V. Terletskaya 1Özge Öztürk Çimentepe Özge Öztürk Çimentepe 6Metin YILDIRIM Metin YILDIRIM 7,8*
  • 1 Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan
  • 2 Department of Chemistry, Faculty of Art and Science, Cukurova University, Adana,, Adana, Türkiye
  • 3 Department of Pharmacy Services, Vocational School of Health Services, Harran University, Sanliurfa, Türkiye, San, Türkiye
  • 4 Dicle University, Faculty of Pharmacy, Department of Analytical Chemistry, 21280, Diyarbakir, Türkiye, Diyarbakır, Türkiye
  • 5 Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Harran University, Sanliurfa, Tü-rkiye, Şanlıurfa, Türkiye
  • 6 Department of Pharmacology, Faculty of Pharmacy, Harran University, Sanliurfa, Türkiye, Şanlıurfa, Türkiye
  • 7 Harran University, Şanlıurfa, Türkiye
  • 8 Department of Biochemistry, Faculty of Pharmacy, Harran University,, Sanliurfa, Türkiye

The final, formatted version of the article will be published soon.

    In this study, Rheum tataricum L. extracts were obtained using various green extraction techniques, including supercritical CO2, subcritical ethanol, and ultrasound-assisted extraction, each performed under optimized parameters. The phytochemical content of the extracts was analyzed using the LC-MS/MS technique, quantifying 53 phytochemicals. Additionally, the in vitro antioxidant properties and antibacterial activities of the extracts were evaluated against Staphylococcus aureus and Enterococcus faecalis as gram-positive bacteria, and Escherichia coli and Pseudomonas aeruginosa as gram-negative bacteria. According to the results, the extracts were rich in catechin, epicatechin, cyranoside, and chlorogenic acid. Extracts obtained via ultrasonic extraction demonstrated stronger antioxidant properties. The IC50 values for the DPPH radical scavenging activity of obtained extracts ranged between 0.0173 mg/mL and 0.0400 mg/mL. The highest total phenolic content was found in the UAE-M-4h extract (213.44 mg GAE/mL). The extracts prepared with UAE-MeOH-2h-4h, UAE-EtOH-2h-4h, Sbc-EtOH-E-140-60-80, Sc-90 atm, and Sc-400 atm showed antibacterial activity against both Grampositive and Gram-negative bacteria at varying rates (MIC range: 31.25 to 250 µg/mL). Based on the all results, the ultrasound assisted extraction proved superior to the other techniques. This study, utilizing three different extraction methods with varying variables such as temperature, pressure, and extraction time, has provided significant insights into which extraction method should be employed for isolating specific phytochemicals or for therapeutic purposes, based on the differing antibacterial results observed. The findings highlight the importance of selecting the appropriate extraction method depending on the target phytochemical or desired antibacterial effect in treatment applications.

    Keywords: Rheum tatarium L., Supercritical carbon dioxide, Subcritical ethanol, Ultrasound assisted extraction, Biological activities

    Received: 19 Oct 2024; Accepted: 13 Nov 2024.

    Copyright: © 2024 Amangeldinova, Ersatır, necip, YILMAZ, Cimentepe, Kudrina, Terletskaya, Öztürk Çimentepe and YILDIRIM. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Nataliya Kudrina, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
    Metin YILDIRIM, Harran University, Şanlıurfa, Türkiye

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