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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Biotechnology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1513438

Isolation and identification of a novel Bacillus velezensis strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae

Provisionally accepted
Xin Zhao Xin Zhao 1Yang Zhai Yang Zhai 1*Lin Wei Lin Wei 2*Fei Xia Fei Xia 3*Yuanru Yang Yuanru Yang 1*Yongjian Yi Yongjian Yi 1*Hongying Wang Hongying Wang 1*Caisheng Qiu Caisheng Qiu 1*Feng Wang Feng Wang 4*Liangbin Zeng Liangbin Zeng 1*
  • 1 Institute of Bast Fiber Crips, Chinese Academy of Agricultural Sciences, Changsha, China
  • 2 Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, Hunan Province, China
  • 3 Central South University Forestry and Technology, Changsha, Hunan Province, China
  • 4 Guizhou Academy of Tobacco Science, Guiyang, China

The final, formatted version of the article will be published soon.

    Bacterial canker is a devastating disease in kiwifruit production, primarily caused by Pseudomonas syringae pv. actinidiae. In this study, a strain of Bacillus velezensis named JIN4, isolated from a kiwifruit branch, showed antagonistic bacterial activity. The B. velezensis JIN4 strain was identified based on its morphological, physiological and biochemical characteristics, 16S rDNA, and gyrA sequences. Furthermore, the complete genome of the strain was elucidated, revealing the presence of three genes that confer resistance to antibiotics, including tetracycline, lincomycin, and streptomycin. Additionally, a cluster of nine secondary metabolite synthesis genes was identified We also determined the whole genome of this strain. In the laboratory, the JIN4 strain exhibited nitrogen (N) and phosphorus (P) production, demonstrating plant growth-promoting and broad-spectrum inhibitory activities against pathogenic fungi and bacteria. In the field, the JIN4 strain demonstrated effective colonization of kiwifruit, increased antioxidant enzyme activity, induced resistance in kiwifruit plants, and achieved a control efficiency of 60.22% against kiwifruit bacterial canker.effectively colonized kiwifruit and increased antioxidant enzyme activity, achieving a 60.22% control efficiency against kiwifruit bacterial canker. These results indicate that B. velezensis JIN4 is a potential biocontrol agent against kiwifruit bacterial canker.

    Keywords: Antibacterial activity, Bacillus velezensis, biocontrol agent, Kiwifruit bacterial canker, whole genome sequencing

    Received: 18 Oct 2024; Accepted: 03 Dec 2024.

    Copyright: © 2024 Zhao, Zhai, Wei, Xia, Yang, Yi, Wang, Qiu, Wang and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yang Zhai, Institute of Bast Fiber Crips, Chinese Academy of Agricultural Sciences, Changsha, China
    Lin Wei, Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, Hunan Province, China
    Fei Xia, Central South University Forestry and Technology, Changsha, 410000, Hunan Province, China
    Yuanru Yang, Institute of Bast Fiber Crips, Chinese Academy of Agricultural Sciences, Changsha, China
    Yongjian Yi, Institute of Bast Fiber Crips, Chinese Academy of Agricultural Sciences, Changsha, China
    Hongying Wang, Institute of Bast Fiber Crips, Chinese Academy of Agricultural Sciences, Changsha, China
    Caisheng Qiu, Institute of Bast Fiber Crips, Chinese Academy of Agricultural Sciences, Changsha, China
    Feng Wang, Guizhou Academy of Tobacco Science, Guiyang, China
    Liangbin Zeng, Institute of Bast Fiber Crips, Chinese Academy of Agricultural Sciences, Changsha, China

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